RT Journal Article
T1 Hypertension alters the function of nitrergic and sensory innervation in mesenteric arteries from female rats
A1 Del Campo Milán, Lara
A1 Ferrer, Mercedes
A1 Balfagón, Gloria
AB Objectives: To investigate whether hypertension could modify the function of adrenergic, nitrergic, and sensory innervations involved in the electrical field stimulation-induced response in mesenteric arteries from female rats.Methods: Vascular reactivity experiments were performed in endothelium-denuded mesenteric arteries from normotensive, Wistar–Kyoto and spontaneously hypertensive female rats; protein expression was measured by western blot; nitric oxide release was measured by fluorometry; calcitonin gene-related peptide and noradrenaline release were determined by enzyme immunoassay.Results: The electrical field stimulation-induced contractions were significantly lower in segments from spontaneously hypertensive rats than those of Wistar–Kyoto rats. Hypertension did not modify either the response or release of noradrenaline. Preincubation with the nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester increased the electrical field stimulation-induced contractions only in segments from Wistar–Kyoto rats. The relaxation induced by the nitric oxide donor sodium nitroprusside was similar in segments from both strains. The electrical field stimulation-induced nitric oxide release was decreased in segments from spontaneously hypertensive rats. The calcitonin gene-related peptide receptor antagonist CGRP(8–37) did not alter the electrical field stimulation-induced contractions in segments from Wistar–Kyoto rats but increased them in segments from spontaneously hypertensive rats. The calcitonin gene-related peptide-induced relaxation was increased in segments from spontaneously hypertensive rats. The expression of the 15-kDa active form of RAMP1 was increased in segments from spontaneously hypertensive rats.Conclusion: In contrast to male rats, electrical field stimulation-induced contractions are decreased in hypertensive female rats. Nitrergic innervation plays a role in the development and/or maintenance of hypertension, whereas sensory innervation is a counteracting mechanism through the increased calcitonin gene-related peptide response.
PB Lippincott Williams & Wilkins
SN 0263-6352
YR 2009
FD 2009
LK https://hdl.handle.net/20.500.14352/96228
UL https://hdl.handle.net/20.500.14352/96228
LA eng
NO del Campo, Lara; Ferrer, Mercedes; Balfagón, Gloria. Hypertension alters the function of nitrergic and sensory innervation in mesenteric arteries from female rats. Journal of Hypertension. 2009 Apr;27(4):791-9.
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Instituto de Salud Carlos III (España)
DS Docta Complutense
RD 26 abr 2025