RT Journal Article
T1 Human rhinovirus‐specific CD8 T cell responses target conserved and unusual epitopes
A1 Gómez Perosanz, Marta
A1 Sánchez Trincado López, José Luis
A1 Fernández Arquero, Miguel
A1 Sidney, John
A1 Sette, Alessandro
A1 Lafuente Duarte, María Esther
A1 Reche Gallardo, Pedro Antonio
AB Human Rhinovirus (HRV) is a major cause of common cold, bronchiolitis, and exacerbations of chronic pulmonary diseases such as asthma. CD8 T cell responses likely play an important role in the control of HRV infection but, surprisingly, HRV-specific CD8 T cell epitopes remain yet to be identified. Here, we approached the discovery and characterization of conserved HRV-specific CD8 T cell epitopes from species A (HRV A) and C (HRV C), the most frequent subtypes in the clinics of various pulmonary diseases. We found IFNγ-ELISPOT positive responses to 23 conserved HRV-specific peptides on peripheral blood mononuclear cells (PBMCs) from 14 HLA I typed subjects. Peptide-specific IFNγ production by CD8 T cells and binding to the relevant HLA I were confirmed for six HRV A-specific and three HRV C-specific CD8 T cell epitopes. In addition, we validated A*02:01-restricted epitopes by DimerX staining and found out that these peptides mediated cytotoxicity. All these A*02:01-restricted epitopes were 9-mers but, interestingly, we also identified and validated an unusually long 16-mer epitope peptide restricted by A*02:01, HRVC1791-1806 (GLEPLDLNTSAGFPYV). HRV-specific CD8 T cell epitopes describe here are expected to elicit CD8 T cell responses in up to 87% of the population and could be key for developing an HRV vaccine.
PB Wiley
SN 0892-6638
YR 2020
FD 2020-11-23
LK https://hdl.handle.net/20.500.14352/116138
UL https://hdl.handle.net/20.500.14352/116138
LA eng
NO Gomez-Perosanz, M., Sanchez-Trincado, J. L., Fernandez-Arquero, M., Sidney, J., Sette, A., Lafuente, E. M., & Reche, P. A. (2021). Human rhinovirus-specific CD8 T cell responses target conserved and unusual epitopes. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(1), e21208. https://doi.org/10.1096/fj.202002165R
NO Ministerio Español de Economı́a y Competitividad (España)
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 5 abr 2025