RT Journal Article
T1 An evaluation of errors in the mitochondrial COI sequencesof Hydrachnidia (Acari, Parasitengona) in public databases
A1 Peláez, María L.
A1 Hórreo Escandón, José Luis
A1 García Jiménez, Ricardo
A1 Valdecasas, Antonio G.
AB Public molecular databases are fundamental tools for modern taxonomic studies whose usefulness rely on the soundness of the data within them. Here, we study potential errors that can arise along the data pipeline from sampling, specimen identifcation and molecular processing (digestion, amplifcation and sequencing) to the submission of sequences to these databases by using the DNA sequences of Hydrachnidia (Acari, Parasitengona) as a case study. Our results indicate that molecular information is available for only about 3% of the Hydrachnidia species known to date; yet, within this small percentage, errors are present in almost 5% of the species analyzed (0.5% of the sequences and almost 11% of the genera). This study underscores the scarcity of genetic data available for Hydrachnidia, but also that the proportion of errors in DNA sequences is relatively small. Even so, it highlights the danger associated with using DNA sequences from public databases, particularly for species identifcation, and reinforces the need for greater quality control measures and/ or protocols to avoid an intensifcation of errors in the (post) genomics era. Finally, our study emphasizes that potential errors may also reveal cryptic diversity within a species.
PB Springer
SN 0168-8162, ESSN: 1572-9702
YR 2022
FD 2022-02-25
LK https://hdl.handle.net/20.500.14352/71713
UL https://hdl.handle.net/20.500.14352/71713
LA eng
NO Gobierno Regional de Asturias
DS Docta Complutense
RD 6 abr 2025