RT Journal Article
T1 The ribonucleolytic activity of the ribotoxin α-sarcin is not essential for in vitro protein biosynthesis inhibition
A1 Alvarez García, Elisa
A1 Diago Navarro, Elizabeth
A1 Herrero Galán, Elías
A1 García Ortega, Lucía
A1 López Villarejo, Juan
A1 Olmo López, Nieves
A1 Díaz Orejas, Ramón
A1 Gavilanes, José G.
A1 Martínez Del Pozo, Álvaro
AB Fungal ribotoxins are toxic secreted ribonucleases that cleave a conserved single phosphodiester bond located at the sarcin/ricin loop of the larger rRNA. This cleavage inactivates ribosomes leading to protein biosynthesis inhibition and cell death. It has been proposed that interactions other than those found at the active site of ribotoxins are needed to explain their exquisite specific activity. The study presented shows the ability of a catalytically inactive α-sarcin mutant (H137Q) to bind eukaryotic ribosomes and interfere with in vitro protein biosynthesis. The results obtained are compatible with previous observations that α-sarcin can promote cell death by a mechanism that is independent of rRNA cleavage, expanding the potential set of activities performed by this family of toxins.
PB Elsevier
SN 1570-9639
YR 2011
FD 2011
LK https://hdl.handle.net/20.500.14352/41964
UL https://hdl.handle.net/20.500.14352/41964
LA spa
NO Ministerio Español de Ciencia e Innovación
NO Ministerio de Educación y Ciencia
NO Gobierno Vasco
NO Federation of European Microbiological Societies
DS Docta Complutense
RD 8 abr 2025