TY - JOUR AU - Lucija Ruzic AU - Bolívar Bolívar, Juan Manuel AU - Bernd Nidetzky PY - 2020 DO - 10.1002/bit.27293 SN - 0006-3592 SN - 1097-0290 UR - https://hdl.handle.net/20.500.14352/114451 AB - The D746E variant of Bifidobacterium bifidum β-N-acetyl-hexosaminidase is a promising glycosynthase (engineered glycosidase deficient in hydrolase activity) for the synthesis of lacto-N-triose II (LNT II), a core structural unit of human milk... LA - eng M2 - 1597 KW - 1,2-oxazoline-activated donor substrate KW - Flow chemistry KW - Glycosynthase KW - Human milk oligosaccharides KW - β-glycosaminidase TI - Glycosynthase reaction meets the flow: Continuous synthesis of lacto‐N‐triose II by engineered β‐hexosaminidase immobilized on solid support TY - journal article VL - 117 ER -