RT Journal Article
T1 Genetic diversity of Mycobacterium avium isolates recovered from clinical samples and from the environment: molecular characterization for diagnostic purposes
A1 Álvarez Sánchez, Julio
A1 Gómez García, Ignacio
A1 Aranaz Martín, Alicia
A1 Bezos Garrido, Javier
A1 Romero Martínez, Beatriz
A1 Juan Ferré, Lucía De
A1 Mateos García, Ana
A1 Gómez Mampaso, Enrique
A1 Domínguez Rodríguez, Lucas José
AB Isolation of Mycobacterium avium complex (MAC) organisms from clinical samples may occur in patients without clinical disease, making the interpretation of results difficult. The clinical relevance of MAC isolates from different types of clinical samples (n = 47) from 39 patients in different sections of a hospital was assessed by comparison with environmental isolates (n = 17) from the hospital. Various methods for identification and typing (commercial probes, phenotypic characteristics, PCR for detection of IS1245 and IS901, sequencing of the hsp65 gene, and pulsed-field gel electrophoresis) were evaluated. The same strain was found in all the environmental isolates, 21 out of 23 (91.3%) of the isolates cultured from urine samples, and 5 out of 19 (26.3%) isolates from respiratory specimens. This strain did not cause disease in the patients. Testing best characterized the strain as M. avium subsp. hominissuis, with the unusual feature that 81.4% of these isolates lacked the IS1245 element. Contamination of certain clinical samples with an environmental strain was the most likely event; therefore, characterization of the environmental mycobacteria present in health care facilities should be performed to discard false-positive isolations in nonsterile samples, mainly urine samples. Molecular techniques applied in this study demonstrated their usefulness for this purpose.
PB American Society for Microbiology
SN 1098-660X
YR 2008
FD 2008-04
LK https://hdl.handle.net/20.500.14352/51964
UL https://hdl.handle.net/20.500.14352/51964
LA eng
NO Ministerio de Ciencia e Innovación (MICINN)
DS Docta Complutense
RD 6 may 2024