RT Journal Article
T1 Immunocytochemical developmental patterns of the thoracolumbar sympathetic chain in the chick and a comparison with its adrenal counterpart
A1 Sánchez-Montesinos, I
A1 Hita-Contreras, F
A1 Espín-Ferra, J
A1 Pasini, B
A1 Mérida Velasco, José Ramón
A1 Rodríguez Vázquez, José Francisco
A1 Cuadra Blanco, Crótida De La
AB The immunocytochemical development of the thoracolumbar sympathetic ganglion and its adrenal counterpart was studied in the chick from days 3.5 to 12 of incubation, using antibodies to 17 separate antigens, including antibodies to pan-neuroendocrine markers, catecholamine-synthesizing and proprotein-processing enzymes, and neuropeptides. Some of the antigens studied (Go protein-alpha subunit, thyrosine hydroxylase, and galanin) were strongly expressed from the first days of development, whereas others (chromogranin-A, chromogranin-B, 7B2 protein, and somatostatin) showed a diverse immunoreactive expression at different stages. Three different patterns were found in the development of both adrenal medulla and thoracolumbar sympathetic ganglion. In the first (chromogranin-A and B, Go protein-alpha subunit, tyrosine hydroxylase, HNK-1, and galanin), virtually all medullary and thoracolumbar sympathetic ganglion cells were strongly immunostained from day 4 onward. Except for HNK-1, chromogranin-A and B, there was a steady increase in immunoreactive cells for all the remaining antigens up to day 12. In the second (7B2 protein, proprotein convertase 2, and secretogranin II), full antigenic expression was reached in medullary and thoracolumbar sympathetic ganglion cells by day 10. In the third pattern (proprotein convertase 3, somatostatin, dopamine-beta-hydroxylase, neuron-specific enolase, vasoactive intestinal polypeptide, and met-enkephalin), differences in immunoreactivity were observed between the medullary and thoracolumbar sympathetic ganglion cells.
PB Sociedad Española de Histología e Ingeniería Tisular
YR 2005
FD 2005
LK https://hdl.handle.net/20.500.14352/112574
UL https://hdl.handle.net/20.500.14352/112574
LA eng
NO Sánchez-Montesinos I, Mérida-Velasco JR, Hita-Contreras F, Espín-Ferra J, Rodríguez-Vázquez JF, De La Cuadra C, Pasini B, Mérida-Velasco JA. Immunocytochemical developmental patterns of the thoracolumbar sympathetic chain in the chick and a comparison with its adrenal counterpart. Histol Histopathol. 2005 Apr;20(2):383-92. doi: 10.14670/HH-20.383. PMID: 15736041.
DS Docta Complutense
RD 31 dic 2025