RT Journal Article
T1 Early in vitro response to Toxoplasma gondii infection in macrophages and neutrophils from sheep immunized with a commercial vaccine
A1 Silva, Marta
A1 Arteche-Villasol, Noive
A1 Criado, Miguel
A1 Ortega Mora, Luis Miguel
A1 Benavides, Julio
A1 Gutiérrez-Expósito, Daniel
AB Although commercial vaccination against ovine toxoplasmosis (Ovilis® Toxovax) has been used to prevent the disease for decades, the mechanisms behind this protection and its effects on target immune cells such as macrophages and neutrophils remain poorly understood. Therefore, peripheral blood monocytes and neutrophils were obtained from vaccinated (n = 5) and non-vaccinated (n = 5) sheep. Ovine monocyte-derived macrophages (OvMØs) were first differentiated in vitro. Neutrophils and OvMØs were inoculated with different T. gondii isolates: TgShSp1 (type II, ToxoDB#3), TgShSp24 (type III, ToxoDB#2) and S48 (type I, Ovilis® Toxovax) and incubated for 3 and 8 h, respectively. Interleukins (ILs) 12, 6, 10 and 1β, along with tumor necrosis factor (TNF), TLR2, TLR8 and macrophage inflammatory protein-1beta (MIP-1β) were significantly upregulated in OvMØs from non-vaccinated sheep compared to vaccinated sheep. However, the transcription levels of iNOS were upregulated in OvMØs from vaccinated ewes. The response of the OvMØs was similar within each group regardless the T. gondii isolate. Similarly, transcription levels of TNF, IL-1β, IL-8, TLR2 and TLR4 were upregulated in neutrophils from non-vaccinated sheep, however no differences in the quantification through fluorimetry of extracellular DNA derived from NETs were observed. These results suggest that vaccination predispose macrophages and neutrophils towards a contained immune response against T. gondii, although it could enhance parasite elimination by macrophages through a higher intracellular production of nitric oxide. Further in vitro studies involving parasite quantification in these cell populations might help to better understand its role in the immune response after vaccination.
PB Elsevier
SN 0165-2427
YR 2026
FD 2026
LK https://hdl.handle.net/20.500.14352/138572
UL https://hdl.handle.net/20.500.14352/138572
LA eng
NO Silva, M., Arteche-Villasol, N., Criado, M., Ortega-Mora, L. M., Benavides, J., & Gutiérrez-Expósito, D. (2026). Early in vitro response to Toxoplasma gondii infection in macrophages and neutrophils from sheep immunized with a commercial vaccine. Veterinary immunology and immunopathology, 299, 111162. Advance online publication. https://doi.org/10.1016/j.vetimm.2026.111162
NO Author contributionD. Gutiérrez-Expósito: Conceptualization, Investigation, Formal analysis, Writing – review & editing, Funding acquisition, Supervision. M.Silva: Investigation, Formal analysis, Writing – original draft, Writing – review & editing. J.Benavides: Conceptualization, Writing – review & editing, Funding acquisition, Supervision. N. Arteche-Villasol: Formal analysis, Writing – review & editing. M. Criado: Writing – review & editing. L.M. Ortega-Mora: Conceptualization, Funding acquisition, Writing – review & editing. All authors have read and approved the final manuscript
NO Ministerio de Economía, Comercio y Empresa (España)
NO European Commission
DS Docta Complutense
RD 20 ago 2026