RT Journal Article
T1 Evaluation of two in vitro pharmacodynamic simulation models: microfiltration versus centrifugation–filtration
A1 Alou Cervera, Luis
A1 Sevillano Fernández, David
A1 Bugella, Javier H.
A1 Fuentes, Fernando
A1 Gómez-Lus Centelles, María Luisa
A1 Prieto Prieto, José
AB Pharmacodynamic in vitro models that simulate serum antimicrobial concentrations provide more information about the activity of an antibiotic than MICs or traditional time-kill methods. The aim of this study was to compare two pharmacodynamic simulation models using ATCC strains of five different species and five antibiotics. In the first model (Centriprep-10 system), a filtration-centrifugation process was used to eliminate the antibiotic; in the second model (microfiltration system) no centrifugation was necessary. The antibiotic concentrations tested were similar to those in serum after normal doses of cefuroxime, clarithromycin, ciprofloxacin, gentamicin and cefotaxime. No significant differences were observed in the killing rates between the models except in the case of Haemophilus influenzae and cefotaxime. The new microfiltration model had the following advantages: lack of the carry-over effect, the absence of centrifugation that could damage bacteria and the possibility of increasing the number of incubation periods to give a better fit of the kinetic profile of man.
PB Elsevier
SN 0924-8579
YR 2001
FD 2001-05
LK https://hdl.handle.net/20.500.14352/107058
UL https://hdl.handle.net/20.500.14352/107058
LA eng
NO Alou L, Sevillano D, Bugella JH, Fuentes F, Gómez-Lus ML, Prieto J. Evaluation of two in vitro pharmacodynamic simulation models: microfiltration versus centrifugation-filtration. Int J Antimicrob Agents. 2001 May;17(5):365-70.
DS Docta Complutense
RD 6 abr 2025