RT Journal Article
T1 Sequence diversity of the internal transcribed spacer (ITS) region of the rRNA operons among different serogroups of Legionella pneumophila isolates
A1 Pérez Luz, Sara
A1 Fernandez, Javier
A1 Rodriguez-Valera, Francisco
A1 Pascual, Leonor
A1 Moreno, Carmen
A1 Amo, Antonio
A1 Apraiz, David
A1 Catalan, Vicente
AB The genus Legionella is represented by 48 species and Legionella pneumophila includes 15 serogroups. In this work, we have studied the intergenic 16S-23S spacer region (ITS) in L. pneumophila to determine the feasability of using amplification polymorphisms in this region, to establish intraspecies differences and to discriminate Legionella species. The amplification of this region, using 16S14F and 23S0R primers, and the analysis of amplicons by the analysis of fragments technique showed that all the L. pneumophila serogroups studied presented the same electrophoretic pattern. Moreover, the analysis of different Legionella species showed that the amplification polymorphisms obtained were species-specific. In order to study the sequence variability of this region, the existence in L. pneumophila of three ribosomal operons was determined by pulsed field gel eletrophoresis (PFGE). Two of the 16S-23S rRNA ITS presented a tRNA Ala and the third one a tRNA Ile. Nevertheless, the variability expected in this region of the operon was not found and the rest of the ITS contained only punctual mutations.
PB Elsevier
SN 0723-2020
YR 2002
FD 2002
LK https://hdl.handle.net/20.500.14352/94584
UL https://hdl.handle.net/20.500.14352/94584
LA eng
NO Pérez-Luz, S., Fernandez, J., Rodríguez-Valera, F., Pascual, L., Moreno, C., Amo, A., Apraiz, D., & Catalán, V. (2002). Sequence diversity of the internal transcribed spacer (ITS) region of the rRNA operons among different serogroups of Legionella pneumophila isolates. Systematic and applied microbiology, 25(2), 212–219. https://doi.org/10.1078/0723-2020-00115
NO Ministerio de Ciencia y Tecnología (España)
NO Ministerio de Industria y Energía (España)
DS Docta Complutense
RD 7 abr 2025