RT Journal Article
T1 Pro-inflammatory and B cell regulating capacities of TWEAK in rainbow trout (Oncorhynchus mykiss)
A1 Abós Gracia, Beatriz
A1 Pérez Fernández, Elena
A1 Morel, Esther
A1 Perdiguero Jiménez, Pedro
A1 Tafalla, Carolina
AB Tumor necrosis factor (TNF)-like weak inducer of apoptosis or TWEAK is a member of the TNF superfamily involved in the regulation of many biological processes. In mammals, TWEAK has been shown to play a role in some autoimmune or inflammatory conditions, but its immune role is not yet clearly defined. In teleost fish, although a few studies have identified homologues to mammalian TWEAK, their biological effects have never been investigated. In the current study, we have studied the transcriptional regulation of two TWEAK homologues (TWEAK 1 and 2) identified in rainbow trout (Oncorhynchus mykiss) throughout different tissues, in response to parasitic or viral infections, or in head kidney (HK) leukocytes stimulated with different stimuli. Although the transcription of both homologues was modulated when HK leukocytes were exposed to several immune stimuli, only TWEAK 1 was significantly modulated upon pathogenic exposure. Thus, we performed a characterization of the functions exerted by this cytokine in HK leukocytes. Recombinant TWEAK 1 strongly up-regulated the transcription of pro-inflammatory genes and antimicrobial peptides in HK leukocytes, with differential transcriptional effects in IgM+ B cells, IgM- lymphocytes and myeloid cells. TWEAK 1 also increased the survival and promoted the differentiation of B cells in HK leukocyte cultures. Our results demonstrate that in teleost fish, TWEAK 1 is involved in the response to different types of pathogens, through the modulation of antimicrobial and pro-inflammatory genes in different leukocytes subsets. Furthermore, a role for TWEAK as a B cell differentiation factor has also been established in rainbow trout.
PB Frontiers 
YR 2021
FD 2021
LK https://hdl.handle.net/20.500.14352/96711
UL https://hdl.handle.net/20.500.14352/96711
LA eng
NO Abós, Beatriz, et al. «Pro-Inflammatory and B Cell Regulating Capacities of TWEAK in Rainbow Trout (Oncorhynchus mykiss)». Frontiers in Immunology, vol. 12, octubre de 2021, p. 748836. https://doi.org/10.3389/fimmu.2021.748836.
NO FundingThis work was supported by the European Research Council (ERC Consolidator Grant 2016 725061 TEMUBLYM) and by the Spanish Ministry of Science, Innovation and Universities (project AGL2017-85494-C2-1-R).
NO European Commission
NO Ministerio de Ciencia, Innovación y Universidades (España)
DS Docta Complutense
RD 7 abr 2025