%0 Journal Article
%A Cuesta Martínez, Ángel
%A Suárez Porto, Eduardo
%A Larsen, Martin
%A Jensen, Kim Bak
%A Sanz, Laura
%A Compte Grau, Marta
%A Kristensen, Peter
%A Álvarez‐Vallina, Luis
%T Enhancement of DNA vaccine potency through linkage of antigen to filamentous bacteriophage coat protein III domain I
%D 2006
%@ 0019-2805
%U https://hdl.handle.net/20.500.14352/101153
%X Although DNA‐based cancer vaccines have been successfully tested in mouse models, a major drawback of cancer vaccination still remains, namely that tumour antigens are weak and fail to generate a vigorous immune response in tumour‐bearing patients. Genetic technology offers strategies for promoting immune pathways by adding immune‐activating genes to the tumour antigen sequence. In this work, we converted a model non‐immunogenic antigen into a vaccine by fusing it to domain I of the filamentous bacteriophage coat protein III gene. Vaccination with a DNA construct encoding the domain I fusion generated antigen‐specific T helper 1‐type cellular immune responses. These results demonstrate that the incorporation of protein III into a DNA vaccine formulation can modulate the gene‐mediated immune response and may thus provide a strategy for improving its therapeutic effect.
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