RT Journal Article
T1 Characterization of a new toxin from the entomopathogenicfungus Metarhizium anisopliae: the ribotoxin anisoplin
A1 Olombrada, Miriam
A1 Medina, Pilar
A1 Budia, Flor
A1 Gavilanes, José
A1 Martínez del Pozo, Álvaro
A1 García Ortega, Lucía
AB Metarhizium anisopliae is an entomopathogenic fungus relevant in biotechnology with applications like malaria vector control. Studies of its virulence factors are therefore of great interest. Fungal ribotoxins are toxic ribonucleases with extraordinary efficiency against target ribosomes and suggested as potential insecticides. Here, we describe this ribotoxin characteristic activity in M. anisopliae cultures. Anisoplin has been obtained as a recombinant protein and further characterized. It is structurally similar to hirsutellin A, the ribotoxin from the entomopathogen Hirsutella thompsonii. Moreover, anisoplin shows the ribonucleolytic activity typical of ribotoxins and cytotoxicity against insect cells. How Metarhizium uses this toxin and possible applications are on perspective.
PB De Gruyter
SN 1431-6730 (Print), 1437-4315 (Online)
YR 2017
FD 2017-01-01
LK https://hdl.handle.net/20.500.14352/17576
UL https://hdl.handle.net/20.500.14352/17576
LA eng
NO Ministerio de Economía y Competitividad (MINECO)
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 30 abr 2024