RT Journal Article
T1 Direct Determination of miR‐21 in Total RNA Extracted from Breast Cancer Samples Using Magnetosensing Platforms and the p19 Viral Protein as Detector Bioreceptor
A1 Torrente Rodríguez, Rebeca Magnolia
A1 Campuzano Ruiz, Susana
A1 López‐Hernández, Eva
A1 Granados, Rosario
A1 Sánchez‐Puelles, José Maria
A1 Pingarrón Carrazón, José Manuel
AB A novel magnetobiosensing approach for the rapid, sensitive and selective miR‐21 detection is reported involving the use of a specific RNA probe (antimiR‐21), streptavidin‐magnetic beads (Strep‐MBs), the siRNA Binding Protein p19 as detector bioreceptor, and amperometric detection with the H2O2/hydroquinone (HQ) system at disposable screen‐printed carbon electrodes. The magnetosensor exhibited a dynamic range from 1.4 to 10 nM and a detection limit of 4.2 fmol of synthetic target miR‐21 without any amplification step in 75 min. The usefulness of the approach was evaluated by analyzing total RNA (RNAt) extracted from metastatic breast cancer cell lines, human tissues and breast cytologies.
PB Wiley
SN 1040-0397
YR 2014
FD 2014
LK https://hdl.handle.net/20.500.14352/96211
UL https://hdl.handle.net/20.500.14352/96211
LA eng
NO Torrente-Rodríguez, R.M., Campuzano, S., López-Hernández, E., Granados, R., Sánchez-Puelles, J.M. and Pingarrón, J.M. (2014), Direct Determination of miR-21 in Total RNA Extracted from Breast Cancer Samples Using Magnetosensing Platforms and the p19 Viral Protein as Detector Bioreceptor. Electroanalysis, 26: 2080-2087. https://doi.org/10.1002/elan.201400317
NO Ministerio de Ciencia e Innovación (España)
NO Ministerio de Economía y Competitividad (España)
NO Comunidad de Madrid
NO European Commission
NO Ministerio de Educación (España)
DS Docta Complutense
RD 7 abr 2025