RT Journal Article
T1 Genotypic Comparison of Pasteurella multocida from Healthy Animals at Entry to the Feedlots with That and from Bovine Respiratory Disease-Affected Animals during the Fattening Period
A1 Calderón Bernal, Johan Manuel
A1 Serna Bernaldo, Carlos
A1 García Muñoz, Ángel
A1 Díez Guerrier, Alberto Antoine
A1 Domínguez Rodríguez, Lucas José
A1 Fernández-Garayzábal Fernández, José Francisco
A1 Vela Alonso, Ana Isabel
A1 Cid Vázquez, María Dolores
AB The aim of this study was to investigate the possible genotypic differences between commensal Pasteurella multocida isolates from apparently healthy animals (AHA) at the time of entry to feedlots and those from BRD-affected animals (BRD-AA). A total of 20 batches of beef calves in seven feedlots were followed-up during the fattening period. P. multocida was isolated from 28.1% of AHA and 22.9% of BRD-AA. All isolates belonged to the A: L3 genotype. Most isolates from clinical cases (81.0%) grouped into a PFGE cluster were significantly associated with BRD cases (OR, 24.9; 95% CI, 6.4–96.2). The whole genomes of 14 isolates representative of the pulsotypes most frequently detected in BRD-AA and AHA were sequenced and compared with 53 bovine genomes belonging to the identified ST13, ST79, and ST80 genotypes for a global comparison. No differences were found in the virulence-associated gene content between sequence types (STs) globally or between BRD-AA and AHA isolates in this study. Significantly, ST79 isolates harbored ARGs, conferring resistance to different antimicrobials, including macrolides and tetracyclines, which are commonly used for the treatment of BRD. Two Spanish ST79 isolates carried an ICE highly similar to ICE Tn7407, which was recently detected in Germany, suggesting that ST79 P. multocida isolates in Europe and North America may be associated with different ICEs.
PB MDPI
SN 2076-2615
YR 2023
FD 2023-08-22
LK https://hdl.handle.net/20.500.14352/103648
UL https://hdl.handle.net/20.500.14352/103648
LA eng
NO Calderón Bernal, J. M., Serna, C., García Muñoz, Á., Díez Guerrier, A., Domínguez, L., Fernández-Garayzábal, J. F., Vela, A. I., & Cid, D. (2023). Genotypic Comparison of Pasteurella multocida from Healthy Animals at Entry to the Feedlots with That and from Bovine Respiratory Disease-Affected Animals during the Fattening Period. Animals : an open access journal from MDPI, 13(17), 2687. https://doi.org/10.3390/ani13172687 Format:
NO Author Contributions: Conceptualization, D.C., A.I.V. and J.F.F.-G.; methodology, D.C., A.I.V. and J.F.F.-G.; software, C.S. and J.F.F.-G.; validation, D.C., A.I.V. and J.F.F.-G.; formal analysis, J.M.C.B. and C.S.; investigation, J.M.C.B. and C.S.; resources, Á.G.M., A.D.G., L.D. and D.C.; data curation, J.M.C.B., C.S., Á.G.M., A.D.G., D.C., A.I.V. and J.F.F.-G.; writing—original draft preparation, D.C. and J.F.F.-G.; writing—review and editing, J.M.C.B., C.S., Á.G.M., A.D.G., D.C., A.I.V., L.D. and J.F.F.-G.; visualization, D.C., A.I.V. and J.F.F.-G.; supervision, D.C.; project administration, D.C.; funding acquisition, D.C. and L.D. All authors have read and agreed to the published version of the manuscript.
NO Universidad Complutense de Madrid
NO Banco de Santander
DS Docta Complutense
RD 5 abr 2025