RT Journal Article
T1 F-actin-binding protein drebrin regulates CXCR4 recruitment to the immune synapse
A1 Pérez-Martínez, Manuel
A1 Gordón-Alonso, Mónica
A1 Román Cabrero, José
A1 Barrero-Villar, Marta
A1 Rey, Mercedes
A1 Mittelbrunn, María
A1 Lamana Domínguez, Amalia
A1 Morlino, Giulia
A1 Calabia, Carmen
A1 Yamazaki, Hiroyuki
A1 Shirao, Tomoaki
A1 Vázquez, Jesús
A1 González-Amaro, Roberto
A1 Veiga, Esteban
A1 Sánchez-Madrid, Francisco
AB The adaptive immune response depends on the interaction of T cells and antigen-presenting cells at the immune synapse. Formation of the immune synapse and the subsequent T-cell activation are highly dependent on the actin cytoskeleton. In this work, we describe that T cells express drebrin, a neuronal actin-binding protein. Drebrin colocalizes with the chemokine receptor CXCR4 and F-actin at the peripheral supramolecular activation cluster in the immune synapse. Drebrin interacts with the cytoplasmic tail of CXCR4 and both proteins redistribute to the immune synapse with similar kinetics. Drebrin knockdown in T cells impairs the redistribution of CXCR4 and inhibits actin polymerization at the immune synapse as well as IL-2 production. Our data indicate that drebrin exerts an unexpected and relevant functional role in T cells during the generation of the immune response.
PB The Company of Biologists
SN 1477-9137
YR 2010
FD 2010
LK https://hdl.handle.net/20.500.14352/96255
UL https://hdl.handle.net/20.500.14352/96255
LA eng
NO Manuel Pérez-Martínez, Mónica Gordón-Alonso, José Román Cabrero, Marta Barrero-Villar, Mercedes Rey, María Mittelbrunn, Amalia Lamana, Giulia Morlino, Carmen Calabia, Hiroyuki Yamazaki, Tomoaki Shirao, Jesús Vázquez, Roberto González-Amaro, Esteban Veiga, Francisco Sánchez-Madrid; F-actin-binding protein drebrin regulates CXCR4 recruitment to the immune synapse. J Cell Sci 1 April 2010; 123 (7): 1160–1170. doi: https://doi.org/10.1242/jcs.064238
NO AcknowledgementsThis work was partly supported by EU-México FONCICYT –C002-2008-1 ALA/127249, SAF-2008-02635, INSINET 01592006 CAM, Red RECAVA RD06/0014-0030 and FIPSE 36658/07 grants. E.V.C. holds a Ramón y Cajal contract from the Spanish Ministry of Science and Innovation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We thank H. de la Fuente for statistical assistance. Editorial assistance was provided by S. Bartlett. G.M. is supported by the European Union-funded International Graduate Program in Molecular Medicine.
NO Consejo Nacional de Humanidades, Ciencias y Tecnologías (México)
NO Ministerio de Ciencia e Innovación (España)
NO European Commission
DS Docta Complutense
RD 11 abr 2025