RT Journal Article
T1 Osteostatin potentiates the bioactivity of mesoporous glass scaffolds containing Zn2+ ions in human mesenchymal stem cell cultures
A1 Heras, Clara
A1 Sánchez Salcedo, Sandra
A1 Lozano Borregón, Daniel
A1 Peña, Juan
A1 Esbrit, Pablo
A1 Vallet Regí, María Dulce Nombre
A1 Salinas Sánchez, Antonio J.
AB There is an urgent need of biosynthetic bone grafts with enhanced osteogenic capacity. In this study, we describe the design of hierarchical meso-macroporous 3D-scaffolds based on mesoporous bioactive glasses (MBGs), enriched with the peptide osteostatin and Zn2+ ions, and their osteogenic effect on human mesenchymal stem cells (hMSCs) as a preclinical strategy in bone regeneration. The MBG compositions investigated were 80%SiO2– 15%CaO–5%P2O5 (in mol-%) Blank (BL), and two analogous glasses containing 4% ZnO (4ZN) and 5% ZnO (5ZN). By using additive fabrication techniques, scaffolds exhibiting hierarchical porosity: mesopores (around 4 nm), macropores (1-600 m) and big channels ( 1000 m), were prepared. These MBG scaffolds with or without osteostatin were evaluated in cell cultures of hMSCs. Zinc promoted hMSCs colonization (both the surface and inside) of MBG scaffolds. Moreover, Zn2+ ions and osteostatin together, but not independently, in the scaffolds were found to induce the osteoblast differentiation genes runt related transcription factor-2 (RUNX2) and alkaline phosphatase (ALP) in hMSCs after 7 d of culture in the absence of an osteogenic differentiation-promoting medium. These results add credence to the combined use of zinc and osteostatin as an effective strategy for bone regeneration applications.
PB Elsevier
SN 1742-7061
YR 2019
FD 2019-03-16
LK https://hdl.handle.net/20.500.14352/13171
UL https://hdl.handle.net/20.500.14352/13171
LA eng
NO RESEARCHER ID N-4501-2014 (Sandra Sánchez Salcedo)ORCID 0000-0002-1889-2057 (Sandra Sánchez Salcedo)RESEARCHER ID B-5081-2017 (Daniel Lozano Borregón)ORCID 0000-0001-5902-9201 (Daniel Lozano Borregón)RESEARCHER ID M-3378-2014 (María Vallet Regí)ORCID 0000-0002-6104-4889 (María Vallet Regí)RESEARCHER ID M-3316-2014 (Antonio Salinas Sánchez)ORCID 0000-0002-8408-3389 (Antonio Salinas Sánchez)
NO Unión Europea. H2020
NO Ministerio de Economía y Competitividad (MINECO)
NO Instituto de Salud Carlos III
DS Docta Complutense
RD 27 abr 2024