RT Journal Article
T1 Dextran Aldehyde in Biocatalysis: More Than a Mere Immobilization System
A1 Tacias-Pascacio, Veymar G.
A1 Ortiz, Claudia
A1 Rueda, Nazzoly
A1 Berenguer-Murcia, Ángel
A1 Acosta Contreras, Niuris
A1 Aranaz Corral, Inmaculada
A1 Civera Tejuca, María Concepción
A1 Fernandez-Lafuente, Roberto
A1 Alcántara León, Andrés Rafael
AB Dextran aldehyde (dexOx), resulting from the periodate oxidative cleavage of 1,2-diol moiety inside dextran, is a polymer that is very useful in many areas, including as a macromolecular carrier for drug delivery and other biomedical applications. In particular, it has been widely used for chemical engineering of enzymes, with the aim of designing better biocatalysts that possess improved catalytic properties, making them more stable and/or active for different catalytic reactions. This polymer possesses a very flexible hydrophilic structure, which becomes inert after chemical reduction; therefore, dexOx comes to be highly versatile in a biocatalyst design. This paper presents an overview of the multiple applications of dexOx in applied biocatalysis, e.g., to modulate the adsorption of biomolecules on carrier surfaces in affinity chromatography and biosensors design, to serve as a spacer arm between a ligand and the support in biomacromolecule immobilization procedures or to generate artificial microenvironments around the enzyme molecules or to stabilize multimeric enzymes by intersubunit crosslinking, among many other applications.
PB MDPI
SN 2073-4344
YR 2019
FD 2019-07-21
LK https://hdl.handle.net/20.500.14352/12660
UL https://hdl.handle.net/20.500.14352/12660
LA eng
NO Ministerio de Ciencia e Innovación (MICINN)
NO Colciencias (Colombia)
DS Docta Complutense
RD 2 may 2024