RT Journal Article
T1 Initiation of leaf somatic embryogenesis involves high pectin esterification, auxin accumulation and DNA demethylation in Quercus alba
A1 Corredoira, Elena
A1 Cano, Vanesa
A1 Barany, Ivett
A1 Solís González, María Teresa
A1 Rodriguez-Sanz, Hector
A1 Vieitez, Ana María
A1 Risueño, María
A1 Testillano, Pilar
AB Somatic embryogenesis is considered a convenient tool for investigating the regulating mechanisms of embryo formation; it is also a feasible system for in vitro regeneration procedures, with many advantages in woody species. Nevertheless, trees have shown recalcitrance to somatic embryogenesis, and its efficiency remains very low in many cases. Consequently, despite the clear potential of somatic embryogenesis in tree breeding programs, its application is limited since factors responsible for embryogenesis initiation have not yet been completely elucidated.In the present work, we investigated key cellular factors involved in the change of developmental program during leaf somatic embryogenesis initiation of white oak (Quercus alba), aiming to identify early markers of the process. The results revealed that pectin esterification, auxin accumulation and DNA demethylation were induced during embryogenesis initiation and differentially found in embryogenic cells, while they were not present in leaf cells before induction or in non-embryogenic cells after embryogenesis initiation. These three factors constitute early markers of leaf embryogenesis and represent processes that could be interconnected and involved in the regulation of cell reprogramming and embryogenesis initiation.These findings provide new insights into the mechanisms underlying plant cell reprogramming, totipotency and embryogenic competence acquisition, especially in tree species for which information is scarce, thus opening up the possibility of efficient manipulation of somatic embryogenesis induction.
PB Elsevier
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/97738
UL https://hdl.handle.net/20.500.14352/97738
LA eng
NO Corredoira, Elena, et al. «Initiation of Leaf Somatic Embryogenesis Involves High Pectin Esterification, Auxin Accumulation and DNA Demethylation in Quercus Alba». Journal of Plant Physiology, vol. 213, junio de 2017, pp. 42-54. https://doi.org/10.1016/j.jplph.2017.02.012.
NO European Commission
NO Ministerio de Economía y Competitividad (España)
DS Docta Complutense
RD 28 abr 2025