RT Journal Article
T1 Neuronal and non-neuronal bradykinin receptors are involved in the contraction and/or relaxation to the pig bladder neck smooth muscle
A1 Fernandes Ribeiro, Ana Sofía
A1 Leite Fernandes, Vitor Samuel
A1 Martínez Sainz, María Del Pilar
A1 Martínez-Sáenz, Ana
A1 Pazos Rodríguez, María Ruth
A1 Orensanz Muñoz, Luis Miguel
A1 Recio Visedo, María Paz
A1 Bustamante Alarma, Salvador
A1 Carballido Rodríguez, Joaquín
A1 García Sacristán, Albino
A1 Prieto Ocejo, Dolores
A1 Hernández Rodríguez, Medardo Vicente
AB Aims: The current study investigates the role played by bradykinin (BK) receptors in the contractility to the pig bladder neck smooth muscle.Methods: Bladder neck strips were mounted in myographs for isometric force recordings and BK receptors expression was also determined by immunohistochemistry.Results: B2 receptor expression was observed in the muscular layer and urothelium whereas B1 expression was consistent detected in urothelium. A strong B2 immunoreactivity was also observed within nerve fibers among smooth muscle bundles. On urothelium-denuded preparations basal tone, BK induced concentration-dependent contractions which were reduced in urothelium-intact samples, by extracellular Ca(2+) removal and by blockade of B2 receptors and voltage-gated Ca(2+) (VOC) and non-VOC channels, and increased by cyclooxygenase (COX) inhibition. On phenylephrine-precontracted denuded strips, under non-adrenergic non-cholinergic (NANC) conditions, electrical field stimulation-elicited frequency-dependent relaxations which were reduced by B2 receptor blockade. In urothelium-intact samples, the B1 receptor agonist kallidin promoted concentration-dependent relaxations which were reduced by blockade of B1 receptors, COX, COX-1 and large-conductance Ca(2+) -activated K(+) (BKCa ) channels and abolished in urothelium-denuded samples and in K(+) -enriched physiological saline solution-precontracted strips.Conclusions: These results suggest that BK produces contraction of pig bladder neck via smooth muscle B2 receptors coupled to extracellular Ca(2+) entry via VOC and non-VOC channels with a minor role for intracellular Ca(2+) mobilization. Facilitatory neuronal B2 receptors modulating NANC inhibitory neurotransmission and urothelial B1 receptors producing relaxation via the COX-1 pathway and BKCa channel opening are also demonstrated.
PB Wiley Periodicals, Inc.
SN 0733-2467
YR 2013
FD 2013-07-12
LK https://hdl.handle.net/20.500.14352/100419
UL https://hdl.handle.net/20.500.14352/100419
LA eng
NO Ribeiro ASF, Fernandes VS, Martínez MP, Martínez-Sáenz A, Pazos MR, Orensanz LM, et al. Neuronal and non-neuronal bradykinin receptors are involved in the contraction and/or relaxation to the pig bladder neck smooth muscle: Bradykinin and Pig Bladder Neck Tension. Neurourol Urodynam 2014;33:558–65. https://doi.org/10.1002/nau.22434.
NO Ministerio de Ciencia e Innovación (España)
NO Fundação para a Ciência e Tecnologia, Ministério da Educação e Ciência (Portugal)
DS Docta Complutense
RD 17 abr 2025