RT Journal Article
T1 Electrochemical immunosensor for sensitive determination of transforming growth factor (TGF) - β1 in urine
A1 Sánchez Tirado, E.
A1 Martínez García, G.
A1 González Cortés, Araceli
A1 Yáñez Sedeño, Paloma
A1 Pingarrón Carrazón, José Manuel
AB The first amperometric immunosensor for the quantification of TGF-β1, a cytokine proposed as a biomarker for patients having or at risk for renal disease, is described in this work. The immunosensor design involves disposable devices using carboxylic acid-functionalized magnetic microparticles supported onto screen-printed carbon electrodes and covalent immobilization of the specific antibody for TGF-β1 using Mix&Go polymer. A sandwich-type immunoassay was performed using biotin-anti-TGF and conjugation with peroxidase-labeled streptavidin (poly-HRP-Strept) polymer. Amperometric measurements were carried out at  0.20 V by adding hydrogen peroxide solution onto the electrode surface in the presence of hydroquinone as the redox mediator. The calibration plot allowed a range of linearity extending between 15 and 3000 pg/mL TGF-β1 which is adequate for the determination of the cytokine in plasma and urine. The limit of detection, 10 pg/mL, is notably improved with respect to those obtained with ELISA kits. The usefulness of the immunosensor for the determination of low TGF-β1 concentrations in real samples was evaluated by analyzing spiked urine at different pg/mL concentration levels.
PB Elsevier
SN ISSN: 0956-5663, ESSN: 1873-4235
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/18770
UL https://hdl.handle.net/20.500.14352/18770
LA eng
NO Ministerio de Economía y Competitividad (MINECO)
NO Comunidad de Madrid
DS Docta Complutense
RD 20 ago 2024