RT Journal Article
T1 Influence of aging and growth hormone on different members of the NFkB family and IkB expression in the heart from a murine model of senescence-accelerated aging
A1 Forman, K.
A1 Vara Ameigeiras, Elena María
A1 García Martín, M. Cruz
A1 Kireev, Roman
A1 Cuesta Sancho, Sara
A1 Acuña Castroviejo, Darío
A1 Fernández-Tresguerres Hernández, Jesús Ángel
AB Inflammation is related to several pathological processes. The aim of this study was to investigate the protein expression of the different subunits of the nuclear factor Kappa b (NFkBp65, p50, p105, p52, p100) and the protein expressions of IkB beta and alpha in the hearts from a murine model of accelerated aging (SAM model) by Western blot. In addition, the translocation of some isoforms of NFkB from cytosol to nuclei (NFkBp65, p50, p52) and ATP level content was studied. In addition we investigated the effect of the chronic administration of growth hormone (GH) on these age-related parameters. SAMP8 and SAMR1 mice of 2 and 10 months of age were used (n = 30). Animals were divided into five experimental groups: 2 old untreated (SAMP8/SAMR1), 2 young control (SAMP8/SAMR1) and one GH treated-old groups (SAMP8). Age-related changes were found in the studied parameters. We were able to see decreases of ATP level contents and the translocation of the nuclear factor kappa B p50, p52 and p65 from cytosol to nuclei in old SAMP8 mice together with a decrease of IKB proteins. However p100 and p105 did not show differences with aging. No significant changes were recorded in SAMR1 animals. GH treatment showed beneficial effects in old SAMP8 mice inducing an increase in ATP levels and inhibiting the translocation of some NFkB subunits such as p52. Our results supported the relation of NFkB activation with enhanced apoptosis and pro-inflammatory status in old SAMP8 mice and suggested a selective beneficial effect of the GH treatment, which was able to partially reduce the incidence of some deleterious changes in the heart of those mice.
PB Elsevier
SN 0531-5565
YR 2016
FD 2016-01
LK https://hdl.handle.net/20.500.14352/100189
UL https://hdl.handle.net/20.500.14352/100189
LA eng
NO Forman K, Vara E, García C, Kireev R, Cuesta S, Acuña-Castroviejo D, Tresguerres JA. Influence of aging and growth hormone on different members of the NFkB family and IkB expression in the heart from a murine model of senescence-accelerated aging. Exp Gerontol. 2016 Jan;73:114-20. doi: 10.1016/j.exger.2015.11.005. Epub 2015 Nov 12. PMID: 26581911.
NO Fundación Rodríguez Pascual de Investigación Medica
NO Fundación Mutua Madrileña
NO Red de envejecimiento y Fragilidad Reticef
NO Instituto Carlos III
DS Docta Complutense
RD 17 abr 2025