RT Journal Article
T1 Effect of growth hormone treatment on pancreatic inflammation, oxidative stress, and apoptosis related to aging in SAMP8 mice
A1 Cuesta Sancho, Sara
A1 Kireev, Roman
A1 García Martín, M. Cruz
A1 Forman, Katherine
A1 Vara Ameigeiras, Elena María
A1 Fernández-Tresguerres Hernández, Jesús Ángel
AB Aging is associated with an increase in inflammation, oxidative stress, and apoptosis. Furthermore, aging is accompanied by an alteration of the growth hormone (GH) -insulin-like growth factor-1 (IGF-1) axis. The aim of this study was to examine the regulation of these parameters in the pancreas of old mice and how GH treatment could affect this process. Male senescence-accelerated prone mice (SAMP8) and male senescence-accelerated resistant mice (SAMR1) 2 (young) and 10 months old were used (n = 40). Animals were divided into five experimental groups: 1 and 2, SAMP8/R1 young control; 3 and 4, SAMP8/R1 old control (untreated); and 5, SAMP8 old treated with GH. Physiologically equivalent doses of GH were administered for 1 month (2 mg subcutaneously [s.c.]/kg/day) and several parameters were analyzed. Aging was associated with increased inflammation, oxidative stress, and apoptosis (increased tumor necrosis factor-α [TNF-α], interleukin-β [IL-β], IL-6, monocyte chemoattractant protein-1 [MCP1], IL-2, heme oxygenase [HO-1], inducible nitric oxide synthase [iNOS], and nitric oxide metabolites [NOx]). The ratio of anti/pro apoptotic mRNA expression-B cell lymphoma 2 (Bcl-2) Bcl-2-associated X protein (BAX) + Bcl-xL/Bcl-2-associated death promoter (BAD)-was decreased during aging in SAMP8 mice. X-inhibitor of apoptosis (XIAP) was decreased during the aging process. Furthermore, no changes were observed in protein expression of nuclear factor-κB (NF-κB p65 and NF-κBp50-105. However, the protein expression of NF-κB p52-100 and inhibitor kappa B (IκB) alpha was increased with age in the pancreas of SAMP8 mice. On the other hand, the expression of IκB beta was decreased with aging. These results indicate that aging is associated with significant alterations in the relative expression of pancreatic genes involved in inflammation, oxidative stress, and apoptosis. According to our results, GH administration to old SAMP8 mice was able to improve pancreas from this parameters.
PB Mary Ann Liebert
SN 1557-8577
YR 2011
FD 2011-10-20
LK https://hdl.handle.net/20.500.14352/99912
UL https://hdl.handle.net/20.500.14352/99912
LA eng
NO Sara Cuesta, Roman Kireev, Cruz García, Katherine Forman, Elena Vara, and Jesús A.F. Tresguerres. Effect of Growth Hormone Treatment on Pancreatic Inflammation, Oxidative Stress, and Apoptosis Related to Aging in SAMP8 Mice. Rejuvenation Research.Oct 2011.501-512.http://doi.org/10.1089/rej.2011.1166
NO Instituto de Salud Carlos III
NO Junta de Andalucía
DS Docta Complutense
RD 4 abr 2025