RT Journal Article
T1 Inflammation determines the pro-adhesive properties of high extracellular D-glucose in human endothelial cells in vitro and rat microvessels in vivo.
A1 Azcutia Criado, Verónica
A1 Abu-Taha, May
A1 Romacho, Tania
A1 Vázquez-Bella, Marta
A1 Matesanz, Nuria
A1 Luscinskas, Francis
A1 Rodríguez-Mañas, Leocadio
A1 Sanz, María Jesús
A1 Sánchez-Ferrer, Carlos
A1 Peiró, Concepción
AB Background: Hyperglycemia is acknowledged as an independent risk factor for developing diabetes-associated atherosclerosis. At present, most therapeutic approaches are targeted at a tight glycemic control in diabetic patients, although this fails to prevent macrovascular complications of the disease. Indeed, it remains highly controversial whether or not the mere elevation of extracellular D-glucose can directly promote vascular inflammation, which favors early proatherosclerotic events. Methods and Findings: In the present work, increasing extracellular D-glucose from 5.5 to 22 mmol/L was neither sufficient to induce intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) expression, analyzed by flow cytometry, nor to promote leukocyte adhesion to human umbilical vein endothelial cells (HUVEC) in vitro, measuredby flow chamber assays. Interestingly, the elevation of D-glucose levels potentiated ICAM-1 and VCAM-1 expression and leukocyte adhesion induced by a pro-inflammatory stimulus, such as interleukin (IL)-1b (5 ng/mL). In HUVEC, high D-glucose augmented the activation of extracellular signal-regulated kinase 1/2 (ERK 1/2) and nuclear transcription factor-kB (NF-kB) elicited by IL-1b, measured by Western blot and electromobility shift assay (EMSA), respectively, but had no effect by itself. Both ERK 1/2 and NF-kB were necessary for VCAM-1 expression, but not for ICAM-1 expression. In vivo, leukocyte trafficking was evaluated in the rat mesenteric microcirculation by intravital microscopy. In accordance with the in vitro data, the acute intraperitoneal injection of D-glucose increased leukocyte rolling flux, adhesion and migration, but only when IL-1b was coadministered.Conclusions: These results indicate that the elevation of extracellular D-glucose levels is not sufficient to promote vascular inflammation, and they highlight the pivotal role of a pro-inflammatory environment in diabetes, as a critical factor conditioning the early pro-atherosclerotic actions of hyperglycemia.
PB Public Library of Science
SN 1932-6203
YR 2010
FD 2010
LK https://hdl.handle.net/20.500.14352/116641
UL https://hdl.handle.net/20.500.14352/116641
LA eng
NO Azcutia V, Abu-Taha M, Romacho T, Vazquez-Bella M, Matesanz N, Luscinskas FW, Rodriguez-Mañas L, Sanz MJ, Sanchez-Ferrer CF, Peiro C: Inflammation Determines the Pro-Adhesive Properties of High Extracellular D-Glucose in Human Endothelial Cells In Vitro and Rat Microvessels In Vivo. PLoS One 2010 Apr 8;5(4):e10091
NO Ministerio de Ciencia y Tecnología (España)
NO Laboratorios Almirall
NO Instituto de Salud Carlos III
NO Fundacion Mutua Madrilena
NO Sociedad Espanola de Farmacologia
DS Docta Complutense
RD 7 abr 2025