RT Journal Article
T1 The Interplay of Mitochondrial Oxidative Stress and Endoplasmic Reticulum Stress in Cardiovascular Fibrosis in Obese Rats
A1 Souza Neto, Francisco V.
A1 Jiménez González, Sara
A1 Delgado Valero, Beatriz
A1 Jurado López, Raquel
A1 Genty, Marie
A1 Romero Miranda, Ana
A1 Rodríguez, Cristina
A1 Nieto, María Luisa
A1 Martínez Martínez, Ernesto
A1 Cachofeiro Ramos, María Victoria
AB We have evaluated the role of mitochondrial oxidative stress and its association with endoplasmic reticulum (ER) stress activation in the progression of obesity-related cardiovascular fibrosis. MitoQ (200 µM) was orally administered for 7 weeks to male Wistar rats that were fed a high-fat diet (HFD, 35% fat) or a control diet (CT, 3.5% fat). Obese animals presented cardiovascular fibrosis accompanied by increased levels of extracellular matrix proteins and profibrotic mediators. These alterations were associated with ER stress activation characterized by enhanced levels (in heart and aorta vs. CT group, respectively) of immunoglobulin binding protein (BiP; 2.1-and 2.6-fold, respectively), protein disulfide-isomerase A6 (PDIA6; 1.9-fold) and CCAAT-enhancer-binding homologous protein (CHOP; 1.5- and 1.8-fold, respectively). MitoQ treatment was able to prevent (p < 0.05) these modifications at cardiac and aortic levels. MitoQ (5 nM) and the ER stress inhibitor, 4-phenyl butyric acid (4 µM), were able to block the prooxidant and profibrotic effects of angiotensin II (Ang II, 10−6 M) in cardiac and vascular cells. Therefore, the data show a crosstalk between mitochondrial oxidative stress and ER stress activation, which mediates the development of cardiovascular fibrosis in the context of obesity and in which Ang II can play a relevant role.
PB MPDI
SN 2076-3921
YR 2021
FD 2021-08-11
LK https://hdl.handle.net/20.500.14352/4872
UL https://hdl.handle.net/20.500.14352/4872
LA eng
NO Souza Neto, F. V., Jiménez González, S., Delgado Valero, B. et al. «The Interplay of Mitochondrial Oxidative Stress and Endoplasmic Reticulum Stress in Cardiovascular Fibrosis in Obese Rats». Antioxidants, vol. 10, n.o 8, agosto de 2021, p. 1274. DOI.org (Crossref), https://doi.org/10.3390/antiox10081274.
NO Instituto de Salud Carlos III/Fondo Europeo de Desarrollo Regional
NO Universidad Complutense de Madrid/Banco de Santander
NO Instituto de Salud Carlos III
NO Comunidad de Madrid
DS Docta Complutense
RD 6 abr 2025