%0 Journal Article
%A Dent, Michael R.
%A López Duarte, Ismael
%A Dickson, Callum J.
%A Chairatana, Phoom
%A Anderson, Harry L.
%A Gould, Ian R.
%A Wylie, Douglas
%A Vyšniauskas, Aurimas
%A Brooks, Nicholas J.
%A Kuimova, Marina K.
%T Imaging plasma membrane phase behaviour in live cells using a thiophene-based molecular rotor
%D 2016
%@ 1359-7345
%@ 1364-548X
%U https://hdl.handle.net/20.500.14352/114908
%X Molecular rotors have emerged as versatile probes of microscopic viscosity in lipid bilayers, although it has proved difficult to find probes that stain both phases equally in phase-separated bilayers. Here, we investigate the use of a membrane-targeting viscosity-sensitive fluorophore based on a thiophene moiety with equal affinity for ordered and disordered lipid domains to probe ordering and viscosity within artificial lipid bilayers and live cell plasma membranes.
%~