RT Journal Article
T1 Biosensing Strategy for Simultaneous and Accurate Quantitative Analysis of Mycotoxins in Food Samples Using Unmodified Graphene Micromotors
A1 Molinero-Fernández, Águeda
A1 Moreno Guzmán, María
A1 López, Miguel Ángel
A1 Escarpa, Alberto
AB A high-performance graphene-based micromotor strategy for simultaneous, fast, and reliable assessment of two highly concerning mycotoxins (fumonisin B1 (FB1) and ocratoxin A (OTA)) has successfully been developed. The assay principle is based on the selective recognition from aptamers to the target mycotoxins and further "on-the-move" fluorescence quenching of the free aptamer in the outer layer of unmodified reduced graphene (rGO; sensing layer) micromotors. Template-prepared rGO/platinum nanoparticles (PtNPs) tubular micromotors were synthesized rapidly and inexpensively by the direct electrodeposition within the conical pores of a polycarbonate template membrane. The new wash-free approach offers using just 1 μL of sample, a simultaneous and rapid "on-the-fly" detection (2 min) with high sensitivity (limits of detection of 7 and 0.4 ng/mL for OTA and FB1, respectively), and high selectivity. Remarkable accuracy (Er < 5%) during the mycotoxin determination in certified reference material as well as excellent quantitative recoveries (96-98%) during the analysis of food samples were also obtained. The excellent results obtained allow envisioning an exciting future for the development of novel applications of catalytic micromotors in unexplored fields such as food safety diagnosis.
PB ACS publication
SN 0003-2700
SN 1520-6882
YR 2017
FD 2017-09-10
LK https://hdl.handle.net/20.500.14352/93020
UL https://hdl.handle.net/20.500.14352/93020
LA eng
NO Anal. Chem. 2017, 89, 20, 10850–10857 Publication Date:September 10, 2017 https://doi.org/10.1021/acs.analchem.7b02440
DS Docta Complutense
RD 29 sept 2024