RT Journal Article
T1 Assessment of diagnostic tools for eradication of bovine tuberculosis in cattle co-infected with Mycobacterium bovis and M. avium subsp. paratuberculosis
A1 Aranaz Martín, Alicia
A1 Juan Ferré, Lucía De
A1 Bezos Garrido, Javier
A1 Álvarez Sánchez, Julio
A1 Romero Martínez, Beatriz
A1 Lozano, Francisco
A1 Paramio, José L.
A1 López-Sánchez, Jesús
A1 Mateos García, Ana Isabel
A1 Domínguez Rodríguez, Lucas José
AB The intradermal tuberculin (IDTB) test and the interferon-gamma (IFN-$\gamma$) assay are used worldwide for detection of bovine tuberculosis in cattle, but little is known about the effect of co-infecting agents on the performance of these diagnostic tests. This report describes a field trial conducted in a cattle herd with dual infection (bovine tuberculosis and paratuberculosis) during 3.5 years. It has been based on a strategic approach encompassing serial parallel testing (comparative IDTB test, the IFN-$\gamma$ assay and serology of paratuberculosis) that was repeated 8 times over the period, and segregation of animals into two herds. The IDTB test detected 65.2% and the IFN-$\gamma$ test detected 69.6% of the Mycobacterium bovis culture-positive cattle. However, the IDTB test performed better during the first part of the trial, while the IFN-$\gamma$ test was the only method that detected infected animals during the following three samplings. The number of false positive reactors with the IDTB and/or the IFN-$\gamma$ tests was remarkably high compared to other reports, and could be caused by cross-reactivity with M. avium subsp. paratuberculosis. Also, the M. bovis isolates from cattle and wildlife from the same property were characterised using molecular techniques to disclose an epidemiological link. The IDTB test may not be appropriate to eradicate bovine tuberculosis in herds with dual mycobacterial infections. This report highlights the need to use several diagnostic techniques for the accurate detection of M. bovis infected animals in these herds.
PB BMC
SN 0928-4249
YR 2006
FD 2006-05-16
LK https://hdl.handle.net/20.500.14352/93610
UL https://hdl.handle.net/20.500.14352/93610
LA eng
NO Aranaz, A., De Juan, L., Bezos, J., Alvarez, J., Romero, B., Lozano, F., Paramio, J. L., López-Sánchez, J., Mateos, A., & Domínguez, L. (2006). Assessment of diagnostic tools for eradication of bovine tuberculosis in cattle co-infected with Mycobacterium bovis and M. avium subsp. paratuberculosis. Veterinary research, 37(4), 593–606. https://doi.org/10.1051/vetres:2006021
NO Corresponding author: Alicia Aranaz alaranaz@vet.ucm.es;Free access;
NO Ministerio de Ciencia y Tecnología
NO Ministerio de Agricultura, Pesca y Alimentación
DS Docta Complutense
RD 6 oct 2024