RT Journal Article
T1 Development of an affordable typing method for Meyerozymaguilliermondii using microsatellite markers
A1 Wrent, Petra
A1 Rivas, Eva María
A1 Martínez Peinado, José
A1 Siloniz Jiménez, María Isabel  de
AB Despite previously published methods, there is still a lack of rapid and affordable methods for genotyping theMeyerozyma guilliermondii yeast species. The development of microsatellite markers is a useful genotyping method in several yeast species. Using the Tandem Repeat Finder Software, a total of 19 microsatellite motifs (di-, tri-, and tetra- repetition) were found in silico in seven of the nine scaffolds published so far. Primer pairs were designed for all of them, although only fourwere used in thiswork. Allmicrosatellite amplifications showed size polymorphism, and the results were identical when repeated. The combination of three microsatellite markers (sc15F/R, sc32 F/R and sc72 F/R) produced a different pattern for each of the Type Culture Collection strains of M. guilliermondii used to optimize the method. The three primer pairs can be used in the same PCR reaction,which reduces costs, in tandem with the fluorescent labeling of only the forward primer in each primer pair.Microsatellite typing was applied on 40 more M. guilliermondii strains. The results showed that no pattern is repeated between the different environmental niches. Four M. guilliermondii strains were only amplified with primer pair sc32 F/R, and subsequently identified as Meyerozyma caribbica by Taq I-RFLP of the 5.8S ITS rDNA. Most out-group species gave negative results even for physiologically similarly species such as Debaryomyces hansenii. The microsatellite markers used in this work were stable over time, which enables their use as a traceability tool.
PB Elsevier
SN 0168-1605
YR 2016
FD 2016-01-09
LK https://hdl.handle.net/20.500.14352/24303
UL https://hdl.handle.net/20.500.14352/24303
LA eng
NO Universidad Complutense de Madrid y Banco Santander
NO Ministerio de Economía y Competitividad (España)
DS Docta Complutense
RD 8 abr 2025