RT Journal Article
T1 Differences in the subgingival microbiome according to stage of periodontitis: a comparison of two geographic regions
A1 Lafaurie, Gloria Inés
A1 Neuta, Yineth
A1 Ríos, Rafael
A1 Pacheco-Montealegre, Mauricio
A1 Pianeta, Roquelina
A1 Castillo, Diana Marcela
A1 Herrera González, David
A1 Reyes, Jinnethe
A1 Diaz, Lorena
A1 Castillo, Yormaris
A1 Sanz Alonso, Mariano
A1 Iniesta Albentosa, Margarita Isabel
AB No microbiological criteria were included in the 2018 EFP-AAP classification of periodontal diseases that could be used to differentiate between stages and grades. Furthermore, differences in the subgingival microbiome depending on stage and grade have not been established. Sixty subgingival biofilm samples were collected in Spain (n = 30) and Colombia (n = 30) from three distinct patient categories: those with periodontal health/gingivitis (n = 20), those with stage I-II periodontitis (n = 20), and those with stage III-IV periodontitis (n = 20). Patients were evaluated by 16S rRNA gene amplification sequencing. Amplicon sequence variants were used to assign taxonomic categories compared to the Human Oral Microbiome Database (threshold ≥97% identity). Alpha diversity was established by Shannon and Simpson indices, and principal coordinate analysis, ANOSIM, and PERMANOVA of the UNIFRAC distances were performed using QIIME2. Although differences in the alpha diversity were observed between samples according to country, Filifactor alocis, Peptostreptococcaceae [XI][G-4] bacterium HMT 369, Fretibacterium fastidiosum, Lachnospiraceae [G-8] bacterium HMT 500, Peptostreptococcaceae [XI][G-5] [Eubacterium] saphenum, Peptostreptococcus stomatis, and Tannerella forsythia were associated with periodontitis sites in all stages. However, only F. alocis, Peptostreptococcaceae [XI][G-4] bacterium HMT 369, Peptostreptococcaceae [XI][G-9] [Eubacterium] brachy, Peptostreptococcaceae [XI][G-5] [Eubacterium] saphenum, and Desulfobulbus sp. HMT 041 were consistent in stage III-IV periodontitis in both countries. Porphyromonas gingivalis and Tannerella forsythia were differentially expressed in severe lesions in the countries studied. Although some non-cultivable microorganisms showed differential patterns between the different stages of periodontitis, they were not the same in the two countries evaluated. Further studies using larger samples with advanced next-generation techniques for high-throughput sequencing of phyla and non-cultivable bacteria within the subgingival microbiome could provide more insight into the differences between stages of periodontitis.
PB Public Library of Science one
YR 2022
FD 2022-08-23
LK https://hdl.handle.net/20.500.14352/113233
UL https://hdl.handle.net/20.500.14352/113233
LA eng
NO Lafaurie GI, Neuta Y, Ríos R, Pacheco-Montealegre M, Pianeta R, Castillo DM, Herrera D, Reyes J, Diaz L, Castillo Y, Sanz M, Iniesta M. Differences in the subgingival microbiome according to stage of periodontitis: A comparison of two geographic regions. PLoS One. 2022 Aug 23;17(8):e0273523. doi: 10.1371/journal.pone.0273523
NO MINCIENCIAS/Colombia (grant number 130880763942)
NO Cátedra Extraordinaria Dentaid de Investigación en Periodoncia, University Complutense of Madrid, Spain
DS Docta Complutense
RD 3 abr 2025