RT Journal Article
T1 The Transcriptional Response of Saccharomyces cerevisiae to Pichia membranifaciens Killer Toxin
A1 Santos de la Sen, Antonio
A1 Álvarez, María del Mar
A1 San Mauro, Manuel
A1 Abrusci, Concepción
A1 Marquina Díaz, Domingo
AB The transcriptional response of Saccharomyces cerevisiae to Pichia membranifaciens killer toxin (PMKT) was investigated. We explored the global gene expression responses of the yeast S. cerevisiae to PMKT using DNA microarrays, real time quantitative PCR, and Northern blot. We identified 146 genes whose expression was significantly altered in response to PMKT in a non-random functional distribution. The majority of induced genes, most of them related to the high osmolarity glycerol (HOG) pathway, were core environmental stress response genes, showing that the coordinated transcriptional response to PMKT is related to changes in ionic homeostasis. Hog1p was observed to be phosphorylated in response to PMKT implicating the HOG signaling pathway. Individually deleted mutants of both up- (99) and down-regulated genes (47) were studied for altered sensitivity; it was observed that the deletion of up-regulated genes generated hypersensitivity (82%) to PMKT. Deletion of down-regulated genes generated wild-type (36%), resistant (47%), and hypersensitive (17%) phenotypes. This is the first study that shows the existence of a transcriptional response to the poisoning effects of a killer toxin.
PB Elsevier
SN 0021-9258
YR 2005
FD 2005-12
LK https://hdl.handle.net/20.500.14352/107870
UL https://hdl.handle.net/20.500.14352/107870
LA eng
NO Santos, A., Del Mar Álvarez, M., Mauro, M. S., Abrusci, C., & Marquina, D. (2005). The transcriptional response of saccharomyces cerevisiae to pichia membranifaciens killer toxin. Journal of Biological Chemistry, 280(51), 41881-41892. https://doi.org/10.1074/jbc.M507014200
DS Docta Complutense
RD 11 abr 2025