RT Journal Article
T1 Proteomic changes related to ‘‘bewildered’’ circulating platelets in the acute coronary syndrome
A1 López Farre, Antonio José
A1 Zamorano León, José Javier
A1 Azcona, Luis
A1 Modrego, Javier
A1 Mateos Cáceres, Petra J.
A1 González Armengol, Juan Jorge
A1 Villaroel, Pedro
A1 Moreno Herrero, Rosario
A1 Rodríguez Sierra, Pablo
A1 Segura, Antonio
A1 Tamargo Menéndez, Juan
A1 Macaya Miguel, Carlos
AB Acute coronary syndromes (ACS) are associated with platelet activation. The aim of the present study was to study the protein expression level associated with glycolysis, oxidative stress, cytoskeleton and cell survival in platelets obtained during an ACS. Platelets from 42 coronary ischemic patients, divided into patients admitted within 24 h after the onset of chest pain (ACS group; n=16) and patients with stable coronary ischemic disease (CAD, n=26), were analyzed using proteomics. The expression levels of proteins involved in cellular cytoskeleton (F-actin capping, β-tubulin, α-tubulin isotypes 1 and 2, vinculin, vimentin and two Ras-related protein Rab-7b isotypes), glycolysis pathway (glyceraldehyde-3-phosphate dehydrogenase, lactate dehydrogenase and two pyruvate kinase isotypes) and cellular-related antioxidant system (manganese superoxide dismutase) and even the expression and activity of glutathione-S-transferase were significantly reduced in platelets from ACS patients compared to CAD patients. Moreover, reduction in the expression of proteins associated with cell survival such as proteasome subunit β type 1 was also observed in ACS platelets compared with CAD platelets. Principal component and logistic regression analysis suggested the existence of factors (proteins) expressed in the platelets inversely associated with acute coronary ischemia. In summary, these results suggest the existence of circulating antioxidant, cytoskeleton and glycolytic-“bewildered” platelets during the acute phase of a coronary event.
PB Wiley
SN 1615-9861
YR 2011
FD 2011-07-01
LK https://hdl.handle.net/20.500.14352/99252
UL https://hdl.handle.net/20.500.14352/99252
LA eng
NO López-Farré AJ, Zamorano-Leon JJ, Azcona L, Modrego J, Mateos-Cáceres PJ, González-Armengol J, Villarroel P, Moreno-Herrero R, Rodríguez-Sierra P, Segura A, Tamargo J, Macaya C. Proteomic changes related to "bewildered" circulating platelets in the acute coronary syndrome. Proteomics. 2011 Aug;11(16):3335-48. doi: 10.1002/pmic.201000708. Epub 2011 Jul 14. PMID: 21751358.
DS Docta Complutense
RD 17 abr 2025