RT Journal Article
T1 Comprehensive analysis of the isomiRome in the vegetative organs of the conifer Pinus pinaster under contrasting water availability
A1 Perdiguero Jiménez, Pedro
A1 Santos Rodrigues, Andreia
A1 Chaves, Inês
A1 Costa, Bruno
A1 Alves, Ana
A1 María, Nuria de
A1 Vélez, María Dolores
A1 Díaz‐Sala, Carmen
A1 Cervera, María Teresa
A1 Miguel, Célia Maria
AB An increasing number of microRNAs (miRNAs) and miRNA-related sequences produced during miRNA biogenesis, comprising the isomiRome, have been recently highlighted in different species as critical mediators of environmental stress responses. Conifers have some of the largest known genomes but an extensive characterization of the isomiRome from any conifer species has been lacking. We provide here a comprehensive overview of the Pinus pinaster isomiRome expressed in roots, stem and needles under well-watered and drought conditions. From the 13,441 unique small RNA sequences identified, 2,980 were annotated as canonical miRNAs or miRNA* and the remaining were classified as isomiRNA or miRNA-like sequences. A survey of their expression patterns highlighted roots as the most responsive organ under drought, where specific sequences of which a 24-nt novel miRNA stood out, were strongly down-regulated. Given the putative roles of the miRNA-targeted transcripts validated specifically in root tissues, some of the miRNAs, conserved and novel, are shortlisted as potential regulators of drought response. These results provide a valuable resource for comparative studies between gymnosperms and angiosperms. Furthermore, it evidences high transferability of the isomiRome between pine species being a useful basis for further molecular regulation and physiological studies, and especially those focused on adaptation to drought conditions.
PB Wiley
SN 0140-7791
YR 2020
FD 2020
LK https://hdl.handle.net/20.500.14352/96639
UL https://hdl.handle.net/20.500.14352/96639
LA eng
NO Perdiguero, Pedro, et al. «Comprehensive Analysis of the isomiRome in the Vegetative Organs of the Conifer Pinus pinaster under Contrasting Water Availability». Plant, Cell & Environment, vol. 44, n.o 3, marzo de 2021, pp. 706-28. https://doi.org/10.1111/pce.13976.
NO Funding information: FP7 People: Marie-Curie Actions, Grant/Award Number: PIEF-GA-2013-627761; Fundação para a Ciência e a Tecnologia, Grant/Award Numbers: IF/01168/2013, SFRH/BD/79779/2011; SFRH/BD/128827/2017; UID/Multi/04551/2013; UID/Multi/04046/2019; DL57/2016/CP1351/CT0003; Horizon 2020 Framework Programme, Grant/Award Number: 676559; Ministerio de Economía, Industria y Competitividad, Gobierno de España, Grant/Award Numbers: AGL2015-66048-C2-1-R, RTI2018-098015-B-I00; Seventh Framework Programme, Grant/Award Number: 289841
NO European Commission
NO Ministerio de Economía y Competitividad (España)
DS Docta Complutense
RD 19 abr 2025