RT Journal Article
T1 Targeting antigens to an invariant epitope of the MHC Class II DR molecule potentiates the immune response to subunit vaccines
A1 Pérez-Filgueira, Mariano
A1 Barderas, María G.
A1 Alonso, Covadonga
A1 Escribano, José M
A1 Gil Dones, Félix
A1 Pastor Vargas, Carlos
A1 Vivanco Martínez, Fernando
AB Recombinant subunit and peptidic vaccines in general present a reduced immunogenicity in vaccinated individuals with respect to the whole pathogen from which they derived. The generation of strong immune responses to these vaccines requires the use of potent adjuvants, high antigen doses and repetitive vaccinations. In this report, we document the enhanced antibody response obtained against two recombinant subunit vaccines by means of targeting to antigen-presenting cells by a recombinant single chain antibody. This antibody, named APCH1, recognizes an epitope of MHC Class II DR molecule preserved in different animal species, including humans. We showed that vaccinal antigens translationally fused to APCH1 antibody and produced by recombinant baculoviruses in insect larvae (Trichoplusia ni), elicited an increased antibody response in comparison with the same antigens alone or fused to a carrier molecule. These results suggest that targeting of antigens to this invariant MHC Class II epitope has immunopotentiating effects that could circumvent the reduced potency of peptidic or subunit vaccines, opening the possibility of widespread application of APCH1 as a new adjuvant antibody of general use.
PB Elsevier
SN 0168-1702
YR 2011
FD 2011
LK https://hdl.handle.net/20.500.14352/105113
UL https://hdl.handle.net/20.500.14352/105113
LA eng
NO Gil F, Pérez-Filgueira M, Barderas MG, Pastor-Vargas C, Alonso C, Vivanco F, Escribano JM. Targeting antigens to an invariant epitope of the MHC Class II DR molecule potentiates the immune response to subunit vaccines. Virus Res. 2011 Jan;155(1):55-60. doi: 10.1016/j.virusres.2010.08.022. Epub 2010 Sep 15. PMID: 20817054.
DS Docta Complutense
RD 16 abr 2025