RT Journal Article
T1 Auranofin-loaded nanoparticles as a new therapeutic tool to fight streptococcal infections
A1 Díez Martínez, Roberto
A1 García Fernández, Esther
A1 Manzano García, Miguel
A1 Martínez, Ángel
A1 Domenech, Mirian
A1 Vallet Regí, María Dulce Nombre
A1 García, Pedro
AB Drug-loaded nanoparticles (NPs) can improve infection treatment by ensuring drug concentration at the right place within the therapeutic window. Poly(lactic-co-glycolic acid) (PLGA) NPs are able to enhance drug localization in target site and to sustainably release the entrapped molecule, reducing the secondary effects caused by systemic antibiotic  administration. We have loaded auranofin, a gold compound traditionally used for treatment of rheumatoid arthritis,into PLGA NPs and their efficiency as antibacterial agent against two Gram-positive pathogens, Streptococcus pneumoniae and Streptococcus pyogenes was evaluated. Auranofin-PLGA NPs showed a strong bactericidal effect as cultures of multiresistant pneumococcal strains were practically sterilized after 6 h of treatment with such auranofin-NPs at 0.25 μM. Moreover, this potent bactericidal effect was also observed in S. pneumoniae and S. pyogenes biofilms, where the same concentration of auranofin-NPs was capable of decreasing the bacterial population about 4 logs more than free auranofin. These results were validated using   zebrafish embryo model demonstrating that treatment with auranofin loaded into NPs achieved a noticeable survival against pneumococcal infections. All these approaches displayed a clear superiority of loaded auranofin PLGA nanocarriers compared to free administration of the drug, which supports their potential application for the treatment of streptococcal infections.
PB Nature Publishing Group
SN 2045-2322
YR 2016
FD 2016-01-18
LK https://hdl.handle.net/20.500.14352/23073
UL https://hdl.handle.net/20.500.14352/23073
LA eng
NO RESEARCHER ID M-3378-2014 (María Vallet Regí)ORCID 0000-0002-6104-4889 (María Vallet Regí)	RESEARCHER ID K-3719-2014  (Miguel Manzano García)RESEARCHER ID M-3378-2014 (Miguel Manzano García)		ORCID 0000-0001-6238-6111 (Miguel Manzano García)Supplementary information accompanies this paper at http://www.nature.com/srepCompeting financial interests: The authors declare no competing financial interests.How to cite this article: Díez-Martínez, R. et al. Auranofin-loaded nanoparticles as a new therapeutic tool tofight streptococcal infections. Sci. Rep. 6, 19525; doi: 10.1038/srep19525 (2016).
NO Ministerio de Economía y Competitividad (MINECO)
NO Ageing Network of Excellence
DS Docta Complutense
RD 10 abr 2025