RT Journal Article
T1 Detection of major food allergens in amniotic fluid: initial allergenic encounter during pregnancy
A1 Pastor Vargas, Carlos
A1 Maroto, Aroa S.
A1 Díaz‐Perales, Araceli
A1 Villalba Díaz, María Teresa
A1 Esteban, Vanesa
A1 Ruiz‐Ramos, Marta
A1 Rodriguez de Alba, Marta
A1 Vivanco Martínez, Fernando
A1 Cuesta‐Herranz, Javier
AB Background: Ingestion of food allergens present in maternal milk during breastfeeding has been hypothesized as a gateway to sensitization to food; however, this process could develop during pregnancy, as the maternal-fetal interface develops a Th2- and Treg-mediated environment to protect the fetus. We hypothesized that in these surroundings, unborn children are exposed to food allergens contained in the mother's diet, possibly giving rise to first sensitization.Methods: The presence of allergens in utero was studied by analyzing amniotic fluid (AF) samples in two different stages of pregnancy: at 15-20 weeks and after delivery at term. An antibody microarray was developed to test for the most common food allergens. The array detects the presence of ten allergens from milk, fruit, egg, fish, nuts, and wheat.Results: AF from 20 pregnant women was collected: eight after delivery at term and 12 from women who underwent diagnostic amniocentesis between weeks 15 and 20 of gestation. The presence of allergens was detected in all samples. Samples from amniocentesis had a higher allergen concentration than samples after delivery at term.Conclusions: We demonstrated the presence of intact major food allergens in AF samples. This early contact could explain subsequent sensitization to foods never eaten before.
PB Wiley
SN 0905-6157
SN 1399-3038
YR 2016
FD 2016-07-29
LK https://hdl.handle.net/20.500.14352/106950
UL https://hdl.handle.net/20.500.14352/106950
LA eng
NO Pastor-Vargas C, Maroto AS, Díaz-Perales A, Villalba M, Esteban V, Ruiz-Ramos M, de Alba MR, Vivanco F, Cuesta-Herranz J. Detection of major food allergens in amniotic fluid: initial allergenic encounter during pregnancy. Pediatr Allergy Immunol. 2016 Nov;27(7):716-720. doi: 10.1111/pai.12608. Epub 2016 Jul 29. PMID: 27341427.
DS Docta Complutense
RD 12 abr 2025