RT Journal Article T1 Trends and Challenges in the Detection and Environmental Surveillance of the Hepatitis E Virus A1 Elois, Mariana Alves A1 Pavi, Catielen Paula A1 Jempierre, Yasmin Ferreira Souza Hoffmann A1 Pilati, Giulia Von Tönnemann A1 Zanchetta, Lucas A1 Grisard, Henrique Borges da Silva A1 García Benzaquén, Nerea A1 Rodríguez-Lázaro, David A1 Fongaro, Gislaine AB The Hepatitis E virus (HEV) is responsible for causing Hepatitis E, a zoonotic disease that has emerged as a significant global health concern, accounting for about 20 million infections and 70,000 deaths annually. Although it is often recognized as a disease that is acute in low-income countries, HEV has also been recognized as a zoonotic disease in high-income countries. The zoonotic transmission requires flexible approaches to effectively monitor the virus, vectors, and reservoirs. However, the environmental monitoring of HEV presents additional challenges due to limitations in current detection methods, making it difficult to accurately assess the global prevalence of the virus. These challenges hinder efforts to fully understand the scope of the disease and to implement effective control measures. This review will explore these and other critical concerns, addressing gaps in HEV research and highlighting the need for improved strategies in the monitoring, prevention, and management of Hepatitis E using a One Health approach. PB MDPI YR 2025 FD 2025 LK https://hdl.handle.net/20.500.14352/120018 UL https://hdl.handle.net/20.500.14352/120018 LA eng NO Elois, M. A., Pavi, C. P., Jempierre, Y. F. S. H., Pilati, G. V. T., Zanchetta, L., Grisard, H. B. d. S., García, N., Rodríguez-Lázaro, D., & Fongaro, G. (2025). Trends and Challenges in the Detection and Environmental Surveillance of the Hepatitis E Virus. Microorganisms, 13(5), 998. https://doi.org/10.3390/microorganisms13050998 NO Author Contributions: Conceptualization, Supervision, G.F. and D.R.-L.; Writing—original draft, tables M.A.E., C.P.P., Y.F.S.H.J., G.V.T.P., L.Z., H.B.d.S.G. and N.G.; Review and editing, M.A.E. All authors have read and agreed to the published version of the manuscript. NO Ministerio de Ciencia, Innovación y Universidades (España) NO Junta de Castilla y León DS Docta Complutense RD 30 dic 2025