RT Journal Article
T1 An electrochemical immunosensor for adiponectin using reduced graphene oxide–carboxymethylcellulose hybrid as electrode scaffold
A1 Arenas Sevillano, Cristian Bernabé
A1 Sánchez Tirado, Esther
A1 Pingarrón Carrazón, José Manuel
A1 Ojeda Fernández, Irene
A1 Gómez Suárez, C.A.
A1 González Cortés, Araceli
A1 Villalonga Santana, Reynaldo
A1 Yáñez-Sedeño Orive, Paloma
AB Reduced graphene oxide–carboxymethylcellulose hybrid (CMC–rGO) was used for the development of a novel electrochemical immunosensor for the determination of adiponectin (APN) cytokine. The hybrid material was synthesized by covalent binding of oxidized CMC to GO layers followed by chemical reduction with sodium borohydride. A sandwich-type immunoassay was employed involving the commercial metal-complexes based polymer Mix & Go™ for the stable and oriented immobilization of anti-APN capture antibody. Biotinylated-anti-APN and HRP-Strept were used for the assay configuration. The APN quantification was performed by amperometry at −200 mV using the hydrogen peroxide/hydroquinone enzyme substrate/mediator system. A sigmoidal calibration plot for APN in the 0.1–50 μg/mL range, with a linear portion in the 0.5–10.0 μg/mL APN concentration range was obtained. The calculated limit of detection (3sb/m) was 61 ng/mL APN. The usefulness of the immunosensor was evaluated by analyzing human serum from hypercholesterolemia or diabetes patients
PB Elsevier
SN 0925-4005
YR 2015
FD 2015
LK https://hdl.handle.net/20.500.14352/95929
UL https://hdl.handle.net/20.500.14352/95929
LA eng
NO Arenas, C. B., Sánchez Tirado, E., Ojeda Fernández, I. et al. «An Electrochemical Immunosensor for Adiponectin Using Reduced Graphene Oxide–Carboxymethylcellulose Hybrid as Electrode Scaffold». Sensors and Actuators B: Chemical, vol. 223, febrero de 2016, pp. 89-94. https://doi.org/10.1016/j.snb.2015.09.055.
NO Ministerio de Economía, Comercio y Empresa (España)
NO Comunidad de Madrid
DS Docta Complutense
RD 21 mar 2026