RT Journal Article
T1 Surfactant dysfunction during over-expression of TGF-β1 precedes profibrotic lung remodeling in vivo
A1 López Rodríguez, Elena
A1 Boden, Caroline
A1 Echaide Torreguitar, Mercedes
A1 Pérez-Gil, Jesús
A1 Kolb, Martin
A1 Gauldie, Jack
A1 Maus, Ulrich A.
A1 Ochs, Matthias
A1 Knudsen, Lars
AB Surfactant dysfunction during overexpression of TGF-1 precedes profibrotic lung remodeling in vivo. Am J Physiol Lung Cell Mol Physiol 310: L1260 –L1271, 2016. First published April 22, 2016; doi:10.1152/ajplung.00065.2016.— Transforming growth factor-1 (TGF-1) is involved in regulation of cellular proliferation, differentiation, and fibrogenesis, inducing myofi- broblast migration and increasing extracellular matrix synthesis. Here, TGF-1 effects on pulmonary structure and function were analyzed. Adenovirus-mediated gene transfer of TGF-1 in mice lungs was performed and evaluated by design-based stereology, invasive pulmonary function testing, and detailed analyses of the surfactant system 1 and 2 wk after gene transfer. After 1 wk decreased static compliance was linked with a dramatic alveolar derecruitment without edema formation or increase in the volume of septal wall tissue or collagen fibrils. Abnormally high surface tension correlated with downregulation of surfactant proteins B and C. TTF-1 expression was reduced, and, using PLA (proximity ligand assay) technology, we found Smad3 and TTF-1 forming complexes in vivo, which are normally translocated into the nucleus of the alveolar epithelial type II cells (AE2C) but in the presence of TGF-1 remain in the cytoplasm. AE2C show altered morphology, resulting in loss of total apical surface area per lung and polarity. These changes of AE2C were progressive 2 wk after gene transfer and correlated with lung compliance. Although static lung compliance remained low, the volume of septal wall tissue and collagen fibrils increased 2 wk after gene transfer. In this animal model, the primary effect of TGF-1 signaling in the lung is downregulation of surfactant proteins, high surface tension, alveolar derecruitment, and mechanical stress, which precede fibrotic tissue remodeling and progressive loss of AE2C polarity. Initial TTF-1 dysfunction is potentially linked to downregulation of surfactant proteins. TGF-1
PB American Physiological Society
SN 1040-0605
YR 2016
FD 2016
LK https://hdl.handle.net/20.500.14352/23388
UL https://hdl.handle.net/20.500.14352/23388
LA eng
NO Ministerio de Economía y Competitividad (España)
NO Comunidad de Madrid
NO Medizinische Hochschule Hannover
NO Alexander von Humboldt Foundation
NO Deutsche Forschungsgemeinschaft
DS Docta Complutense
RD 25 dic 2025