RT Journal Article
T1 Effect of paratuberculosis on the diagnosis of bovine tuberculosis in a cattle herd with a mixed infection using interferon-gamma detection assay
A1 Álvarez Sánchez, Julio
A1 Juan Ferré, Lucía De
A1 Bezos Garrido, Javier
A1 Romero Martínez, Beatriz
A1 Sáez, Jose Luis
A1 Marqués, Sergio
A1 Domínguez, Concepción
A1 Mínguez, Olga
A1 Fernández-Mardomingo, Baudilio
A1 Mateos García, Ana Isabel
A1 Domínguez Rodríguez, Lucas José
A1 Aranaz Martín, Alicia
AB Interferon-gamma (IFN-gamma) detection assay is being applied as an ancillary test to tuberculin tests in the diagnosis of bovine tuberculosis to detect the maximum number of infected animals. Among possible factors influencing the performance of tuberculosis-diagnostic tests, paratuberculosis, a widespread disease in Spain and other European countries, has been pointed out as a cause of false positive reactions. Still, its effect on the sensitivity of these tests in cattle has yet to be fully characterized. The impact of paratuberculosis in the apparent sensitivity of IFN-gamma assay was studied in a bullfighting cattle herd with a mixed tuberculosis-paratuberculosis infection, using culture of Mycobacterium bovis and Mycobacterium avium paratuberculosis as the gold standard to determine the infection status of every animal. A total of 218 animals were slaughtered and sampled for bacteriology after blood sampling. IFN-gamma assay showed a lower apparent sensitivity in animals with a mixed infection (50%) compared to all animals suffering tuberculosis (78.3%). This finding indicates that the presence of paratuberculosis in tuberculosis-infected herds could imply a serious impairment in the sensitivity of IFN-gamma detection test.
PB Elsevier
SN 0378-1135
YR 2009
FD 2009
LK https://hdl.handle.net/20.500.14352/93331
UL https://hdl.handle.net/20.500.14352/93331
LA eng
NO Álvarez, Julio, et al. «Effect of Paratuberculosis on the Diagnosis of Bovine Tuberculosis in a Cattle Herd with a Mixed Infection Using Interferon-Gamma Detection Assay». Veterinary Microbiology, vol. 135, n.o 3-4, marzo de 2009, pp. 389-93. https://doi.org/10.1016/j.vetmic.2008.09.060.
NO Ministerio de Ciencia y Tecnología (España)
NO European Commission
NO Ministerio de Agricultura, Pesca y Alimentación (España)
NO Ministerio de Educación y Cultura (España)
DS Docta Complutense
RD 27 dic 2025