%0 Journal Article
%A Gómez Mejía, Esther
%A Hartwig Mikkelsen, Line
%A Rosales Conrado, Noelia
%A León González, María Eugenia de
%A Madrid Albarrán, María Yolanda
%T A combined approach based on matrix solid-phase dispersion extraction assisted by titanium dioxide nanoparticles and liquid chromatography to determine polyphenols from grape residues
%D 2021
%@ 0021-9673
%U https://hdl.handle.net/20.500.14352/93590
%X A simple and efficient low-cost matrix solid phase dispersion (MSPD) extraction assisted by TiO2  nanopar- ticles and diatomaceous earth has been developed for the extraction of phenolic compounds from grape and grape pomace wastes. Experimental conditions for MSPD extraction were optimized by a facto- rial design and a surface response methodology. The simultaneous identification and quantification of eight main natural polyphenols (caffeic, p-coumaric, dihydroxybenzoic and gallic acid, rutin, resveratrol, quercetin and catechin) was possible by combining MSPD and capillary liquid chromatography coupled to a diode array detection and a mass simple quadrupole analyzer (cLC-DAD-MS). Good linearity and acceptable LOD (0.05–62 μg· g −1 ) and LOQ (0.2–207 μg· g −1 ) were obtained. The quantities of extracted polyphenols were within 2.4 and 333 μg· g −1,  with catechin and rutin the most abundant compounds in rape pomace and grape wastes, respectively. Furthermore, considering the prospective uses of the win- ery bioresidues, the extracts have been characterised in terms of bioactive properties (several antioxidant activities and bacterial inhibition against Staphylococcus aureus, Escherichia coli and Pseudomona aerugi- nosa)  and parameters such as total polyphenol and total flavonoid content. The high antioxidant activity (IC 50 5.0 ± 0.4 μg ·g −1 against DPPH radical) and antibacterial activity (2.2 ± 0.3 mg· mL −1 ) suggests that the methodology developed is efficient, rapid and promising for the extraction of phenolic compounds with potential application as bioactive ingredients in food and cosmetic industries.
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