RT Journal Article
T1 A combined approach based on matrix solid-phase dispersion extraction assisted by titanium dioxide nanoparticles and liquid chromatography to determine polyphenols from grape residues
A1 Gómez Mejía, Esther
A1 Hartwig Mikkelsen, Line
A1 Rosales Conrado, Noelia
A1 León González, María Eugenia de
A1 Madrid Albarrán, María Yolanda
A2 Elsevier, 
AB A simple and efficient low-cost matrix solid phase dispersion (MSPD) extraction assisted by TiO2  nanopar- ticles and diatomaceous earth has been developed for the extraction of phenolic compounds from grape and grape pomace wastes. Experimental conditions for MSPD extraction were optimized by a facto- rial design and a surface response methodology. The simultaneous identification and quantification of eight main natural polyphenols (caffeic, p-coumaric, dihydroxybenzoic and gallic acid, rutin, resveratrol, quercetin and catechin) was possible by combining MSPD and capillary liquid chromatography coupled to a diode array detection and a mass simple quadrupole analyzer (cLC-DAD-MS). Good linearity and acceptable LOD (0.05–62 μg· g −1 ) and LOQ (0.2–207 μg· g −1 ) were obtained. The quantities of extracted polyphenols were within 2.4 and 333 μg· g −1,  with catechin and rutin the most abundant compounds in rape pomace and grape wastes, respectively. Furthermore, considering the prospective uses of the win- ery bioresidues, the extracts have been characterised in terms of bioactive properties (several antioxidant activities and bacterial inhibition against Staphylococcus aureus, Escherichia coli and Pseudomona aerugi- nosa)  and parameters such as total polyphenol and total flavonoid content. The high antioxidant activity (IC 50 5.0 ± 0.4 μg ·g −1 against DPPH radical) and antibacterial activity (2.2 ± 0.3 mg· mL −1 ) suggests that the methodology developed is efficient, rapid and promising for the extraction of phenolic compounds with potential application as bioactive ingredients in food and cosmetic industries.
PB Elsevier
SN 0021-9673
YR 2021
FD 2021
LK https://hdl.handle.net/20.500.14352/93590
UL https://hdl.handle.net/20.500.14352/93590
LA eng
NO Gómez-Mejía, E; Mikkelsen, LH; Rosales-Conrado, N; León-González, ME; Madrid, Y, A combined approach based on matrix solid-phase dispersion extraction assisted by titanium dioxide nanoparticles and liquid chromatography to determine polyphenols from grape residues, 2021 May 10; 1644; 462128
NO Comunidad de Madrid
NO Ministerio de Economía y Competitividad (España)
NO Universidad Complutense de Madrid
NO European Commission
DS Docta Complutense
RD 30 may 2025