RT Journal Article
T1 Electroanalytical immunotool to determine matricellular protein periostin, a stromal biomarker of prognosis in colorectal cancer
A1 Blázquez García, Marina
A1 Quinchia, Jennifer
A1 Ruiz Valdepeñas Montiel, Víctor
A1 Torrente Rodríguez, Rebeca Magnolia
A1 Serafín González-Carrato, Verónica
A1 Garranzo Asensio, María
A1 García Romero, Ana
A1 Orozco, Jahir
A1 Barderas Manchado, Rodrigo
A1 Pingarrón Carrazón, José Manuel
A1 Campuzano Ruiz, Susana
AB Tumor-associated stroma biomarkers are emerging as key signaling molecules of metastasis in colorectal cancer (CRC). In this sense, periostin (POSTN), a protein of the extracellular matrix from the stromal compartment, is envisioned as a potential stromal prognostic biomarker, which facilitates the application of pertinent treatments. In this work, we report an easy-to-handle amperometric sandwich-based immunosensing strategy for the determination of POSTN involving commercial magnetic microparticles (MBs), disposable carbon electrodes, and the horseradish peroxidase (HRP)/H2O2/hydroquinone (HQ) electrochemical system. The method allowed a dynamic linear range between 0.47 and 25 ng mL−1 and a limit of detection, LOD, of 0.14 ng mL−1 compatible with clinical demands. The method was applied to the analysis of POSTN in a variety of cancer-related real bio-scenarios including cell extracts and secretomes from CRC cells, and plasma from CRC patients at different stages. The potentials of this firstly developed MBs-assisted immunoplatform are justified by distinguishing between metastatic abilities of cultured CRC cells through the analysis of their extracts and secretomes, and by differentiating between healthy controls and CRC patients in just 60 min. Therefore, the developed immunoplatform can be envisaged as a novel and profitable tool for exploring the most uncharacterized but prospective tumor areas.
PB Chemistry Europe
YR 2023
FD 2023-12-22
LK https://hdl.handle.net/20.500.14352/123872
UL https://hdl.handle.net/20.500.14352/123872
LA eng
NO lázquez‐García, Marina, et al. «Electroanalytical Immunotool to Determine Matricellular Protein Periostin, a Stromal Biomarker of Prognosis in Colorectal Cancer». ChemElectroChem, vol. 11, n.o 3, febrero de 2024, p. e202300641. DOI.org (Crossref), https://doi.org/10.1002/celc.202300641.
NO Ministerio de Ciencia, Innovación y Universidades
NO Universidad Complutense de Madrid
NO Ministerio de Comercio, Industria y Turismo
NO Instituto Colombiano de Crédito Educativo y Estudios Técnicos en el Exterior
NO Universidad de Antioquia
DS Docta Complutense
RD 1 ene 2026