RT Journal Article
T1 Evaluation of polyesteramide (PEA) and polyester (PLGA) microspheres as intravitreal drug delivery systems in albino rats
A1 Peters, Tobias
A1 Kim, Seong-Woo
A1 Castro, Vinicius
A1 Stingl, Krunoslav
A1 Strasser, Torsten
A1 Bolz, Sylvia
A1 Schraermeyer, Ulrich
A1 Mihov, George
A1 Zong, MengMeng
A1 Andrés Guerrero, Vanesa
A1 Herrero Vanrell, María Del Rocío
A1 Dias, Aylvin
A1 Cameron, Neil
A1 Zrenner, Eberhart
AB Purpose: To study the suitability of injectable microspheres based on poly(ester amide) (PEA) or poly lactic-co-glycolic acid (PLGA) as potential vehicles for intravitreal drug delivery in rat eyes. Dexamethasone loaded PEA microspheres (PEA+DEX) were also evaluated.Methods: Forty male Sprague Dawley rats were divided into four groups that received different intravitreally injected microspheres: PEA group (n=12); PLGA group (n=12); PEA+DEX group (n=8); and control group (no injection, n=8). Electroretinography (ERG), fundus autofluorescence (FAF), and spectral domain optical coherence tomography (sdOCT) were performed at baseline, weeks 1 and 2, and months 1, 2, and 3 after intravitreal injection. Eyes were histologically examined using light microscopy and transmission electron microscopy at the end of the in vivo study.Results: There were no statistically significant changes in ERG among the groups. Abnormal FAF pattern and abnormal deposits in OCT were observed after injection but almost completely disappeared between week 2 and month 3 in all injected groups. GFAP staining showed that Müller glia cell activation was most pronounced in PLGA-injected eyes. Increased cell death was not observed by TUNEL staining at month 1. In electron microscopy at month 3, the remnants of microparticles were found in the retinal cells of all injected groups, and loss of plasma membrane was seen in the PLGA group.Conclusions: Although morphological changes such as mild glial activation and material remnants were observed histologically 1 month and 3 months after injection in all injected groups, minor cell damage was noted only in the PLGA group at 3 months after injection. No evidence of functional abnormality relative to untreated eyes could be detected by ERG 3 months after injection in all groups. Changes observed in in vivo imaging such as OCT and FAF disappeared after 3 months in almost all cases.
PB Elsevier
SN 0142-9612
YR 2017
FD 2017-04
LK https://hdl.handle.net/20.500.14352/18744
UL https://hdl.handle.net/20.500.14352/18744
LA eng
NO Peters, T. Kim, S., Castro, V. et al. «Evaluation of Polyesteramide (PEA) and Polyester (PLGA) Microspheres as Intravitreal Drug Delivery Systems in Albino Rats». Biomaterials, vol. 124, abril de 2017, pp. 157-68. DOI.org (Crossref), https://doi.org/10.1016/j.biomaterials.2017.02.006.
NO Unión Europea
NO Tistou and Charlotte Kerstan Foundation
DS Docta Complutense
RD 7 oct 2024