RT Journal Article
T1 Immunoneuroendocrine, Stress, Metabolic, and Behavioural Responses in High-Fat Diet-Induced Obesity
A1 Navarro, María del Carmen
A1 Gálvez, Isabel
A1 Hinchado, Isabel
A1 Otero, Eduardo
A1 Torres Piles, Silvia
A1 Francisco Morcillo, Javier
A1 Fuente Del Rey, María Mónica De La
A1 Martín Cordero, Leticia
A1 Ortega, Eduardo
AB Obesity has reached global epidemic proportions, and even though its effects are well-documented, studying the interactions among all influencing factors is crucial for a better understanding of its physiopathology. In a high-fat-diet-induced obesity animal model using C57BL/6J mice, behavioural responses were assessed through a battery of tests, while stress biomarkers and systemic inflammatory cytokines were measured using an Enzyme-Linked ImmunoSorbent Assay and a Bio-Plex Multiplex System. The peritoneal macrophage microbicide capacity was analysed via flow cytometry, and crown-like structures (CLSs) in white adipose tissue (WAT) were evaluated through staining techniques. Results indicated that obese mice exhibited increased body weight, hyperglycaemia, and hyperlipidaemia after 18 weeks on a high-fat diet, as well as worse physical conditions, poorer coordination and balance, and anxiety-like behaviour. Differences in corticosterone and noradrenaline concentrations were also found in obese animals, revealing a stress response and noradrenergic dysregulation, along with a weakened innate immune response characterized by a lower microbicide capacity, and the presence of an underlying inflammation evidenced by more CLSs in WAT. Altogether, these findings indicate that obesity deteriorates the entire stress, inflammatory, metabolic, sensorimotor and anxiety-like behavioural axis. This demonstrates that jointly evaluating all these aspects allows for a deeper and better exploration of this disease and its associated comorbidities, emphasizing the need for individualized and context-specific strategies for its management.
PB MDPI
SN 2072-6643
YR 2024
FD 2024
LK https://hdl.handle.net/20.500.14352/118012
UL https://hdl.handle.net/20.500.14352/118012
LA eng
NO Navarro, M.d.C.; Gálvez, I.; Hinchado, M.D.; Otero, E.; Torres-Piles, S.; Francisco-Morcillo, J.; de La Fuente, M.; Martín-Cordero, L.; Ortega, E. Immunoneuroendocrine, Stress, Metabolic, and Behavioural Responses in High-Fat Diet-Induced Obesity. Nutrients 2024, 16, 2209. https://doi.org/10.3390/nu16142209
NO This research was funded by the Gobierno de Extremadura-Fondo Europeo de Desarrollo Regional, Spain (GR21079; IB18011).
NO Gobierno de Extremadura
NO European Commission
DS Docta Complutense
RD 21 abr 2025