RT Journal Article
T1 HIV transgene expression impairs K+ channel function in the pulmonary vasculature
A1 Mondejar Parreño, Gema
A1 Morales Cano, Daniel
A1 Barreira, Bianca
A1 Callejo Arranz, María
A1 Ruiz-Cabello Osuna, Jesús
A1 Moreno Gutiérrez, Laura
A1 Esquivel Ruiz, Sergio Antonio
A1 Mathie, Alistair
A1 Butrous, Ghazwan
A1 Pérez Vizcaíno, Francisco
A1 Cogolludo Torralba, Ángel Luis
AB Human immunodeficiency virus (HIV) infection is an established risk factor for pulmonary arterial hypertension (PAH); however, the pathogenesis of HIV-related PAH remains unclear. Since K+ channel dysfunction is a common marker in most forms of PAH, our aim was to analyze whether the expression of HIV proteins is associated with impairment of K+ channel function in the pulmonary vascular bed. HIV transgenic mice (Tg26) expressing seven of the nine HIV viral proteins and wild-type (WT) mice were used. Hemodynamic assessment was performed by echocardiography and catheterization. Vascular reactivity was studied in endothelium-intact pulmonary arteries. K+ currents were recorded in freshly isolated pulmonary artery smooth muscle cells (PASMC) using the patch-clamp technique. Gene expression was assessed using quantitative RT-PCR. PASMC from Tg26 mice had reduced K+ currents and were more depolarized than those from WT. Whereas voltage-gated K+ channel 1.5 (Kv1.5) currents were preserved, pH-sensitive noninactivating background currents (IKN) were nearly abolished in PASMC from Tg26 mice. Tg26 mice had reduced lung expression of Kv7.1 and Kv7.4 channels and decreased responses to the Kv7.1 channel activator L-364,373 assessed by vascular reactivity and patch-clamp experimental approaches. Although we found pulmonary vascular remodeling and endothelial dysfunction in Tg26 mice, this was not accompanied by changes in hemodynamic parameters. In conclusion, the expression of HIV proteins in vivo impairs pH-sensitive IKN and Kv7 currents. This negative impact of HIV proteins in K+ channels was not sufficient to induce PAH, at least in mice, but may play a permissive or accessory role in the pathophysiology of HIV-associated PAH.
PB American Physiological Society
SN 1040-0605
SN 1522-1504
YR 2018
FD 2018-11-01
LK https://hdl.handle.net/20.500.14352/97445
UL https://hdl.handle.net/20.500.14352/97445
LA eng
NO Mondejar-Parreño G, Morales-Cano D, Barreira B, Callejo M, Ruiz-Cabello J, Moreno L, Esquivel-Ruiz S, Mathie A, Butrous G, Perez-Vizcaino F, Cogolludo A. HIV transgene expression impairs K+ channel function in the pulmonary vasculature. Am J Physiol Lung Cell Mol Physiol. 2018 Nov 1;315(5):L711-L723. doi: 10.1152/ajplung.00045.2018
DS Docta Complutense
RD 10 abr 2025