RT Journal Article
T1 Sensitive Monitoring of Enterobacterial Contamination of Food Using Self-Propelled Janus Microsensors
A1 Pacheco, Marta
A1 Jurado-Sánchez, Beatriz
A1 Escarpa, Alberto
AB Food poisoning caused by bacteria is a major cause of disease and death worldwide. Herein we describe the use of Janus micromotors as mobile sensors for the detection of toxins released by enterobacteria as indicators of food contamination. The micromotors are prepared by a Pickering emulsion approach and rely on the simultaneous encapsulation of platinum nanoparticles for enhanced bubble-propulsion and receptor-functionalized quantum dots (QDs) for selective binding with the 3-deoxy-d-manno-oct-2-ulosonic acid target in the endotoxin molecule. Lipopolysaccharides (LPS) from Salmonella enterica were used as target endotoxins, which upon interaction with the QDs induce a rapid quenching of the native fluorescence of the micromotors in a concentration-dependent manner. The micromotor assay can readily detect concentrations as low as 0.07 ng mL–1 of endotoxin, which is far below the level considered toxic to humans (275 μg mL–1). Micromotors have been successfully applied for the detection of Salmonella toxin in food samples in 15 min compared with several hours required by the existing Gold Standard method. Such ultrafast and reliable approach holds considerable promise for food contamination screening while awaiting the results of bacterial cultures in a myriad of food safety and security defense applications.
PB American Chemical Society
SN 0003-2700
YR 2018
FD 2018-01-27
LK https://hdl.handle.net/20.500.14352/93654
UL https://hdl.handle.net/20.500.14352/93654
LA eng
NO M. Pacheco, B. Jurado Sánchez, A. Escarpa*. Sensitive monitoring of enterobacterial contamination of food using self-propelled Janus microsensors. Analytical Chemistry, 90 (2018), 2912-2917.
NO University of Alcala
NO Spanish Ministry of Education
NO Spanish Ministry of Economy and Competitiveness
NO NANOAVANSENS
DS Docta Complutense
RD 4 abr 2025