RT Journal Article
T1 Staphylococcus aureus CC398: host adaptation and emergence of methicillin resistance in livestock
A1 Price, Lance B
A1 Stegger, Marc
A1 Hasman, Henrik
A1 Aziz, Maliha
A1 Larsen, Jesper
A1 Andersen, Paal Skytt
A1 Pearson, Talima
A1 Waters, Andrew E
A1 Foster, Jeffrey T
A1 Schupp, James
A1 Gillece, John
A1 Driebe, Elizabeth
A1 Liu, Cindy M
A1 Springer, Burkhard
A1 Zdovc, Irena
A1 Battisti, Antonio
A1 Franco, Alessia
A1 Zmudzki, Jacek
A1 Schwarz, Stefan
A1 Butaye, Patrick
A1 Jouy, Eric
A1 Pomba, Constanca
A1 Porrero, M Concepción
A1 Ruimy, Raymond
A1 Smith, Tara C
A1 Robinson, D Ashley
A1 Weese, J Scott
A1 Arriola, Carmen Sofia
A1 Yu, Fangyou
A1 Laurent, Frederic
A1 Keim, Paul
A1 Skov, Robert
A1 Aarestrup, Frank M
AB UNLABELLEDSince its discovery in the early 2000s, methicillin-resistant Staphylococcus aureus (MRSA) clonal complex 398 (CC398) has become a rapidly emerging cause of human infections, most often associated with livestock exposure. We applied whole-genome sequence typing to characterize a diverse collection of CC398 isolates (n = 89), including MRSA and methicillin-susceptible S. aureus (MSSA) from animals and humans spanning 19 countries and four continents. We identified 4,238 single nucleotide polymorphisms (SNPs) among the 89 core genomes. Minimal homoplasy (consistency index = 0.9591) was detected among parsimony-informative SNPs, allowing for the generation of a highly accurate phylogenetic reconstruction of the CC398 clonal lineage. Phylogenetic analyses revealed that MSSA from humans formed the most ancestral clades. The most derived lineages were composed predominantly of livestock-associated MRSA possessing three different staphylococcal cassette chromosome mec element (SCCmec) types (IV, V, and VII-like) including nine subtypes. The human-associated isolates from the basal clades carried phages encoding human innate immune modulators that were largely missing among the livestock-associated isolates. Our results strongly suggest that livestock-associated MRSA CC398 originated in humans as MSSA. The lineage appears to have undergone a rapid radiation in conjunction with the jump from humans to livestock, where it subsequently acquired tetracycline and methicillin resistance. Further analyses are required to estimate the number of independent genetic events leading to the methicillin-resistant sublineages, but the diversity of SCCmec subtypes is suggestive of strong and diverse antimicrobial selection associated with food animal production.IMPORTANCEModern food animal production is characterized by densely concentrated animals and routine antibiotic use, which may facilitate the emergence of novel antibiotic-resistant zoonotic pathogens. Our findings strongly support the idea that livestock-associated MRSA CC398 originated as MSSA in humans. The jump of CC398 from humans to livestock was accompanied by the loss of phage-carried human virulence genes, which likely attenuated its zoonotic potential, but it was also accompanied by the acquisition of tetracycline and methicillin resistance. Our findings exemplify a bidirectional zoonotic exchange and underscore the potential public health risks of widespread antibiotic use in food animal production.
PB American Society for Microbiology
SN 2150-7511
YR 2012
FD 2012
LK https://hdl.handle.net/20.500.14352/45171
UL https://hdl.handle.net/20.500.14352/45171
LA eng
DS Docta Complutense
RD 25 abr 2025