RT Journal Article T1 Sensitive detection of major food allergens in breast milk: first gateway for allergenic contact during breastfeeding A1 Pastor Vargas, Carlos A1 Maroto, Aroa A1 Díaz‐Perales, Araceli A1 Villalba Díaz, María Teresa A1 Casillas Diaz, Natalia A1 Vivanco Martínez, Fernando A1 Cuesta‐Herranz, Javier AB Food allergy is recognized as a major public health issue, especially in early childhood. It has been hypothesized that early sensitization to food allergens maybe due to their ingestion as components dissolved in the milk during the breastfeeding, explaining reaction to a food, which has never been taken before. Thus, the aim of this work has been to detect the presence of the food allergens in breast milk by microarray technology. We produced a homemade microarray with antibodies produced against major food allergens. The antibody microarray was incubated with breast milk from 14 women collected from Fundación Jiménez Díaz Hospital. In this way, we demonstrated the presence of major foods allergens in breast milk. The analysis of allergens presented in breast milk could be a useful tool in allergy prevention and could provide us a key data on the role of this feeding in tolerance induction or sensitization in children. PB Wiley SN 0105-4538 YR 2015 FD 2015 LK https://hdl.handle.net/20.500.14352/94358 UL https://hdl.handle.net/20.500.14352/94358 LA eng NO Pastor-Vargas C, Maroto AS, Díaz-Perales A, Villaba M, Casillas Diaz N, Vivanco F, Cuesta-Herranz J. Sensitive detection of major food allergens in breast milk: first gateway for allergenic contact during breastfeeding. Allergy. 2015 Aug;70(8):1024-7. doi: 10.1111/all.12646. Epub 2015 May 27. PMID: 25952012. NO Ministerio de Sanidad (España) DS Docta Complutense RD 7 jul 2025