RT Journal Article
T1 Formoterol decreases muscle wasting as well as inflammation in the rat model of rheumatoid arthritis
A1 Gómez San Miguel, Ana Belén
A1 Gómez Moreira, Carolina
A1 Nieto Bona, María Paz
A1 Fernández Galaz, María Del Carmen
A1 Villanúa Bernués, María Ángeles
A1 Martín Velasco, Ana Isabel
A1 López-Calderón Barreda, Asunción
AB Adjuvant-induced arthritis is an experimental model of rheumatoid arthritis that is associated with body weight loss and muscle wasting. β2-adrenergic receptor agonists are powerful anabolic agents that trigger skeletal muscle hypertrophy and have been proposed as a promising treatment for muscle wasting in human patients. The aim of this work was to determine whether formoterol, a selective β2-adrenoreceptor agonist, is able to ameliorate muscle wasting in arthritic rats. Arthritis was induced in male Wistar rats by intradermal injection of Freund's adjuvant. Control and arthritic rats were injected daily with 50 μg/kg sc formoterol or saline for 12 days. Body weight change, food intake, and arthritis index were analyzed. After euthanasia, in the gastrocnemius mRNA was analyzed by PCR, and proteins were analyzed by Western blotting. Arthritis decreased gastrocnemius weight, cross-sectional area, and myofiber size, whereas formoterol increased those variables in both arthritic and control rats. Formoterol decreased the external signs of arthritis as well as NF-κB(p65) activation, TNFα, and COX-2 levels in the gastrocnemius of arthritic and control rats. Those effects of formoterol were associated with a decreased expression of myostatin, atrogin-1, and MuRF1 and in LC3b lipidation. Arthritis increased the expression of MyoD, myogenin, IGF-I, and IGFBP-3 and -5 in the gastrocnemius. In control and in arthritic rats, treatment with formoterol increased Akt phosphorylation and myogenin levels, whereas it decreased IGFBP-3 expression in the gastrocnemius. These data suggest that formoterol has an anti-inflammatory effect and decreases muscle wasting in arthritic rats through increasing Akt activity and myogenin and decreasing myostatin, the p-NF-κB(p65)/TNF pathway, and IGFBP-3.
PB American Physiological Society
SN 1522-1555
YR 2016
FD 2016-06-01
LK https://hdl.handle.net/20.500.14352/91692
UL https://hdl.handle.net/20.500.14352/91692
LA eng
NO Gómez-SanMiguel AB, Gomez-Moreira C, Nieto-Bona MP, Fernández-Galaz C, Villanúa MÁ, Martín AI, López-Calderón A. Formoterol decreases muscle wasting as well as inflammation in the rat model of rheumatoid arthritis. Am J Physiol Endocrinol Metab. 2016 Jun 1;310(11):E925-37. doi: 10.1152/ajpendo.00503.2015. PMID: 27245339.
NO MINECO
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 17 abr 2025